Mice deficient in Six5 develop cataracts: Implications for myotonic dystrophy

Todd R. Klesert, Diane H. Cho, John I. Clark, James Maylie, John Adelman, Lauren Snider, Eric C. Yuen, Philippe Soriano, Stephen J. Tapscott

Research output: Contribution to journalArticlepeer-review

200 Scopus citations


Expansion of a CTG trinucleotide repeat in the 3' UTR of the gene DMPK at the DM1 locus on chromosome 19 causes myotonic dystrophy a dominantly inherited disease characterized by skeletal muscle dystrophy and myotonia, cataracts and cardiac conduction defects. Targeted deletion of Dm15, the mouse orthologue of human DMPK, produced mice with a mild myopathy and cardiac conduction abnormalities, but without other features of myotonic dystrophy, such as myotonia and cataracts. We, and others, have demonstrated that repeat expansion decreases expression of the adjacent gene SIX5 (refs 7,8), which encodes a home-odomain transcription factor. To determine whether SIX5 deficiency contributes to the myotonic dystrophy phenotype, we disrupted mouse Six5 by replacing the first exon with a β-galactosidase reporter. Six5-mutant mice showed reporter expression in multiple tissues, including the developing lens. Homozygous mutant mice had no apparent abnormalities of skeletal muscle function, but developed lenticular opacities at a higher rate than controls. Our results suggest that SIX5 deficiency contributes to the cataract phenotype in myotonic dystrophy, and that myotonic dystrophy represents a multigenic disorder.

Original languageEnglish (US)
Pages (from-to)105-109
Number of pages5
JournalNature genetics
Issue number1
StatePublished - May 2000

ASJC Scopus subject areas

  • Genetics


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