Microscopic computed tomography-based skeletal phenotyping for genetic model organisms

Suresh I. Prajapati, Lisa Nevell, Charles Keller

Research output: Chapter in Book/Report/Conference proceedingChapter


Forward and reverse genetics now enable researchers to understand embryonic and postnatal gene functioning in a wide range of species. Some genetic mutations cause obvious morphological change, whereas other mutations can lead to more subtle phenotypes and might be overlooked without adequate observations and quantifications. Due to the increase in number of genetic model organisms examined by the growing field of phenomics, standardized but sensitive methods for quantitative analysis are increasingly necessary in the everyday practice of analyzing ever-increasing quantities of phenotypic data. In this chapter, we have presented platform-independent parameters for the use of microscopic X-ray computed tomography (microCT) for phenotyping species-specific skeletal morphology of a variety of different genetic model organisms.

Original languageEnglish (US)
Title of host publicationMouse Molecular Embryology
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Number of pages6
ISBN (Print)9781603272902
StatePublished - 2014

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Embryogenesis
  • Genetic model organisms
  • Skeletal phenotyping
  • microCT

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics


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