TY - JOUR
T1 - Mitochondrial replacement therapy in reproductive medicine
AU - Wolf, Don P.
AU - Mitalipov, Nargiz
AU - Mitalipov, Shoukhrat
N1 - Funding Information:
This work was supported by grants from National Institutes of Health R01-HD063276, R01-HD057121, R01-HD059946, R01-EY021214, P51-OD011092, a grant from the Leducq Foundation, and OHSU institutional funds.
Publisher Copyright:
© 2014 Elsevier Ltd.
PY - 2015/2/1
Y1 - 2015/2/1
N2 - Mitochondrial dysfunction is implicated in disease and age-related infertility. Mitochondrial replacement therapies (MRT) in oocytes or zygotes, such as pronuclear (PNT), spindle (ST), or polar body (PBT) transfer, could prevent second-generation transmission of mitochondrial DNA (mtDNA) defects. PNT, associated with high levels of mtDNA carryover in mice but low levels in human embryos, carries ethical issues secondary to donor embryo destruction. ST, developed in primates, supports normal development to adults and low mtDNA carryover. PBT in mice, coupled with PN or ST, may increase the yield of reconstructed embryos with low mtDNA carryover. MRT also offers replacement of the deficient cytoplasm in oocytes from older patients, with the expectation of high pregnancy rates following in vitro fertilization.
AB - Mitochondrial dysfunction is implicated in disease and age-related infertility. Mitochondrial replacement therapies (MRT) in oocytes or zygotes, such as pronuclear (PNT), spindle (ST), or polar body (PBT) transfer, could prevent second-generation transmission of mitochondrial DNA (mtDNA) defects. PNT, associated with high levels of mtDNA carryover in mice but low levels in human embryos, carries ethical issues secondary to donor embryo destruction. ST, developed in primates, supports normal development to adults and low mtDNA carryover. PBT in mice, coupled with PN or ST, may increase the yield of reconstructed embryos with low mtDNA carryover. MRT also offers replacement of the deficient cytoplasm in oocytes from older patients, with the expectation of high pregnancy rates following in vitro fertilization.
KW - Female infertility
KW - Mitochondria
KW - Mitochondrial DNA
KW - Mitochondrial replacement therapy
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U2 - 10.1016/j.molmed.2014.12.001
DO - 10.1016/j.molmed.2014.12.001
M3 - Review article
C2 - 25573721
AN - SCOPUS:84922947421
SN - 1471-4914
VL - 21
SP - 68
EP - 76
JO - Trends in Molecular Medicine
JF - Trends in Molecular Medicine
IS - 2
ER -