Mitochondrial replacement therapy in reproductive medicine

Don P. Wolf, Nargiz Mitalipov, Shoukhrat Mitalipov

Research output: Contribution to journalReview articlepeer-review

125 Scopus citations

Abstract

Mitochondrial dysfunction is implicated in disease and age-related infertility. Mitochondrial replacement therapies (MRT) in oocytes or zygotes, such as pronuclear (PNT), spindle (ST), or polar body (PBT) transfer, could prevent second-generation transmission of mitochondrial DNA (mtDNA) defects. PNT, associated with high levels of mtDNA carryover in mice but low levels in human embryos, carries ethical issues secondary to donor embryo destruction. ST, developed in primates, supports normal development to adults and low mtDNA carryover. PBT in mice, coupled with PN or ST, may increase the yield of reconstructed embryos with low mtDNA carryover. MRT also offers replacement of the deficient cytoplasm in oocytes from older patients, with the expectation of high pregnancy rates following in vitro fertilization.

Original languageEnglish (US)
Pages (from-to)68-76
Number of pages9
JournalTrends in Molecular Medicine
Volume21
Issue number2
DOIs
StatePublished - Feb 1 2015

Keywords

  • Female infertility
  • Mitochondria
  • Mitochondrial DNA
  • Mitochondrial replacement therapy

ASJC Scopus subject areas

  • Molecular Medicine
  • Molecular Biology

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