TY - JOUR
T1 - Mn(II) oxidation in Pseudomonas putida GB-1 is influenced by flagella synthesis and surface substrate
AU - Geszvain, Kati
AU - Yamaguchi, Ai
AU - Maybee, Jared
AU - Tebo, Bradley M.
N1 - Funding Information:
Acknowledgments This work was supported by grant number MCB-0630355 from the National Science Foundation. The contents of the manuscript are solely the responsibility of the authors and do not necessarily represent the official views of the National Science Foundation. We also thank Karen Visick for helpful advice and stimulating discussion.
PY - 2011/8
Y1 - 2011/8
N2 - Bacterially mediated manganese(II) oxidation greatly affects the biogeochemical cycling of Mn and other elements. One species of bacteria that are capable of Mn(II) oxidation is the gamma-proteobacterium Pseudomonas putida GB-1. In this organism, Mn(II) oxidation begins in stationary phase on the outer surface of the cell, forming a layer of insoluble Mn(III,IV) oxides. A random transposon mutagenesis screen isolated 12 mutant strains of P. putida GB-1 that exhibited increased Mn(II) oxidation on solid media relative to wild type. In 8 out of the 12 strains, the transposon had inserted into a putative flagellar gene. Those 8 strains each had motility defects, thus the disrupted genes are part of the P. putida GB-1 flagellar regulon. The flagellar genes identified include putative structural components (FliC, FliD, FlgE, and FlgL) and regulatory proteins (FlgM and FleN). Deletion of either the FleN gene (fleN) or the overlapping gene fliA resulted in increased Mn(II) oxidation, while in-frame deletion of fliF, which encodes an essential component of the basal body, did not. In liquid media, the flagellar mutants exhibited delayed Mn(II) oxidation relative to wild type. These results suggest that bacterial Mn(II) oxidation is regulated in part by flagellar-mediated responses to the surface substrate.
AB - Bacterially mediated manganese(II) oxidation greatly affects the biogeochemical cycling of Mn and other elements. One species of bacteria that are capable of Mn(II) oxidation is the gamma-proteobacterium Pseudomonas putida GB-1. In this organism, Mn(II) oxidation begins in stationary phase on the outer surface of the cell, forming a layer of insoluble Mn(III,IV) oxides. A random transposon mutagenesis screen isolated 12 mutant strains of P. putida GB-1 that exhibited increased Mn(II) oxidation on solid media relative to wild type. In 8 out of the 12 strains, the transposon had inserted into a putative flagellar gene. Those 8 strains each had motility defects, thus the disrupted genes are part of the P. putida GB-1 flagellar regulon. The flagellar genes identified include putative structural components (FliC, FliD, FlgE, and FlgL) and regulatory proteins (FlgM and FleN). Deletion of either the FleN gene (fleN) or the overlapping gene fliA resulted in increased Mn(II) oxidation, while in-frame deletion of fliF, which encodes an essential component of the basal body, did not. In liquid media, the flagellar mutants exhibited delayed Mn(II) oxidation relative to wild type. These results suggest that bacterial Mn(II) oxidation is regulated in part by flagellar-mediated responses to the surface substrate.
KW - Biofilm
KW - Flagella
KW - Mn(II) oxidation
KW - Motility
KW - Pseudomonas putida GB-1
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U2 - 10.1007/s00203-011-0702-0
DO - 10.1007/s00203-011-0702-0
M3 - Article
C2 - 21479918
AN - SCOPUS:79960898785
SN - 0302-8933
VL - 193
SP - 605
EP - 614
JO - Archives of Microbiology
JF - Archives of Microbiology
IS - 8
ER -