Modulation of calcium-activated potassium small conductance (SK) current in rat dopamine neurons of the ventral tegmental area

Kush Paul; Dove J. Keith; Steven Johnson

doi:10.1016/S0304-3940(03)00747-X

Modulation of calcium-activated potassium small conductance (SK) current in rat dopamine neurons of the ventral tegmental area

Kush Paul, Dove J. Keith, Steven Johnson

Brenden-Colson Center for Pancreatic Health

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Block of calcium-sensitive potassium SK current (gKCa_[SK]) by apamin or bicuculline methiodide potentiates burst firing in dopamine neurons in the presence of N-methyl-D-aspartate (NMDA). The purpose of this study was to test the hypothesis that calcium entry through NMDA-gated channels can potentiate gKCa_[SK] in dopamine neurons in the ventral tegmental area. We used microelectrodes to record an outward tail current that was evoked by membrane depolarization under single-electrode voltage-clamp. Using bicuculline methiodide (50 μM) as a reversible inhibitor of gKCa _[SK], we found that NMDA (15 μM) reduced the peak amplitude of the outward tail current by 39%. Contrary to expectations, our results suggest that stimulation of NMDA receptors reduces the calcium-activated potassium gKCa_[SK], an effect that could facilitate NMDA-dependent burst firing.

Original language	English (US)
Pages (from-to)	180-184
Number of pages	5
Journal	Neuroscience Letters
Volume	348
Issue number	3
DOIs	https://doi.org/10.1016/S0304-3940(03)00747-X
State	Published - Sep 18 2003

Keywords

Apamin
Bicuculline methiodide
Brain slice
Ca dependent potassium SK channel
Dopamine
Ventral tegmental area

ASJC Scopus subject areas

Neuroscience(all)

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10.1016/S0304-3940(03)00747-X

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title = "Modulation of calcium-activated potassium small conductance (SK) current in rat dopamine neurons of the ventral tegmental area",

abstract = "Block of calcium-sensitive potassium SK current (gKCa[SK]) by apamin or bicuculline methiodide potentiates burst firing in dopamine neurons in the presence of N-methyl-D-aspartate (NMDA). The purpose of this study was to test the hypothesis that calcium entry through NMDA-gated channels can potentiate gKCa[SK] in dopamine neurons in the ventral tegmental area. We used microelectrodes to record an outward tail current that was evoked by membrane depolarization under single-electrode voltage-clamp. Using bicuculline methiodide (50 μM) as a reversible inhibitor of gKCa [SK], we found that NMDA (15 μM) reduced the peak amplitude of the outward tail current by 39%. Contrary to expectations, our results suggest that stimulation of NMDA receptors reduces the calcium-activated potassium gKCa[SK], an effect that could facilitate NMDA-dependent burst firing.",

keywords = "Apamin, Bicuculline methiodide, Brain slice, Ca dependent potassium SK channel, Dopamine, Ventral tegmental area",

author = "Kush Paul and Keith, {Dove J.} and Steven Johnson",

note = "Funding Information: This study was supported by National Institutes of Mental Health Grant MH40416 (S.W.J.), the National Alliance for Research on Schizophrenia and Depression (K.P.) and the Portland Parkinson's Disease Research Education and Clinical Center (S.W.J).",

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doi = "10.1016/S0304-3940(03)00747-X",

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AU - Paul, Kush

AU - Keith, Dove J.

AU - Johnson, Steven

N1 - Funding Information: This study was supported by National Institutes of Mental Health Grant MH40416 (S.W.J.), the National Alliance for Research on Schizophrenia and Depression (K.P.) and the Portland Parkinson's Disease Research Education and Clinical Center (S.W.J).

PY - 2003/9/18

Y1 - 2003/9/18

N2 - Block of calcium-sensitive potassium SK current (gKCa[SK]) by apamin or bicuculline methiodide potentiates burst firing in dopamine neurons in the presence of N-methyl-D-aspartate (NMDA). The purpose of this study was to test the hypothesis that calcium entry through NMDA-gated channels can potentiate gKCa[SK] in dopamine neurons in the ventral tegmental area. We used microelectrodes to record an outward tail current that was evoked by membrane depolarization under single-electrode voltage-clamp. Using bicuculline methiodide (50 μM) as a reversible inhibitor of gKCa [SK], we found that NMDA (15 μM) reduced the peak amplitude of the outward tail current by 39%. Contrary to expectations, our results suggest that stimulation of NMDA receptors reduces the calcium-activated potassium gKCa[SK], an effect that could facilitate NMDA-dependent burst firing.

AB - Block of calcium-sensitive potassium SK current (gKCa[SK]) by apamin or bicuculline methiodide potentiates burst firing in dopamine neurons in the presence of N-methyl-D-aspartate (NMDA). The purpose of this study was to test the hypothesis that calcium entry through NMDA-gated channels can potentiate gKCa[SK] in dopamine neurons in the ventral tegmental area. We used microelectrodes to record an outward tail current that was evoked by membrane depolarization under single-electrode voltage-clamp. Using bicuculline methiodide (50 μM) as a reversible inhibitor of gKCa [SK], we found that NMDA (15 μM) reduced the peak amplitude of the outward tail current by 39%. Contrary to expectations, our results suggest that stimulation of NMDA receptors reduces the calcium-activated potassium gKCa[SK], an effect that could facilitate NMDA-dependent burst firing.

KW - Apamin

KW - Bicuculline methiodide

KW - Brain slice

KW - Ca dependent potassium SK channel

KW - Dopamine

KW - Ventral tegmental area

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Modulation of calcium-activated potassium small conductance (SK) current in rat dopamine neurons of the ventral tegmental area

Abstract

Keywords

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