@article{281522f83ed94994821b5e87e92335d0,
title = "Molecular architecture of the chick vestibular hair bundle",
abstract = "Hair bundles of the inner ear have a specialized structure and protein composition that underlies their sensitivity to mechanical stimulation. Using mass spectrometry, we identified and quantified >1,100 proteins, present from a few to 400,000 copies per stereocilium, from purified chick bundles; 336 of these were significantly enriched in bundles. Bundle proteins that we detected have been shown to regulate cytoskeleton structure and dynamics, energy metabolism, phospholipid synthesis and cell signaling. Three-dimensional imaging using electron tomography allowed us to count the number of actin-actin cross-linkers and actin-membrane connectors; these values compared well to those obtained from mass spectrometry. Network analysis revealed several hub proteins, including RDX (radixin) and SLC9A3R2 (NHERF2), which interact with many bundle proteins and may perform functions essential for bundle structure and function. The quantitative mass spectrometry of bundle proteins reported here establishes a framework for future characterization of dynamic processes that shape bundle structure and function.",
author = "Shin, {Jung Bum} and Krey, {Jocelyn F.} and Ahmed Hassan and Zoltan Metlagel and Tauscher, {Andrew N.} and Pagana, {James M.} and Sherman, {Nicholas E.} and Jeffery, {Erin D.} and Spinelli, {Kateri J.} and Hongyu Zhao and Wilmarth, {Phillip A.} and Dongseok Choi and David, {Larry L.} and Manfred Auer and Barr-Gillespie, {Peter G.}",
note = "Funding Information: high-pressure freezing and imaging; D. Jorgens provided mentoring in high-pressure freezing. We would like to thank A. Cheng, B. Carragher and C. Potter for help with electron microscopy data collection at the National Resource for Automated Molecular Microscopy, supported by US National Institutes of Health (NIH) National Center for Research Resources grant RR017573. For technical assistance, we acknowledge A. Snyder of the Advanced Light Microscopy Core at The Jungers Center (Oregon Health & Science University), supported by shared instrumentation grants S10 RR023432 and S10 RR025440 from the National Center for Research Resources (NIH). Work described here was supported by NIH grants K99/R00 DC009412 (J.B.S.), F32 DC012455 (J.F.K.), R01 DC002368 (P.G.B.-G.), R01 DC011034 (P.G.B.-G.), P30 DC005983 (P.G.B.-G.), R01 EY007755 (L.L.D.), P30 EY10572 (L.L.D.) and P01 GM051487 (M.A.).",
year = "2013",
month = mar,
doi = "10.1038/nn.3312",
language = "English (US)",
volume = "16",
pages = "365--374",
journal = "Nature Neuroscience",
issn = "1097-6256",
publisher = "Nature Publishing Group",
number = "3",
}