Molecular biology of antibiotic production in bacillus

Michiko M. Nakano; Peter Zuber

doi:10.3109/07388559009038209

Molecular biology of antibiotic production in bacillus

Michiko M. Nakano
, Peter Zuber

Research output: Contribution to journal › Article › peer-review

84 Scopus citations

Abstract

Several species of the genus Bacillus produce peptide antibiotics which are synthesized either through a ribosomal or non-ribosomal mechanism. The antibiotics gramicidin, tyrocidine, and bacitracin are synthesized nonribosomally by the multienzyme thiotemplate mechanism. Surfactin and mycobacillin are also synthesized nonribosomally but by a mechanism that, apparently, is distinct from that of the multienzyme thiotemplate. Other antibiotics such as subtilin are gene encoded and are ribosomally synthesized. Molecular genetic and DNA sequence analysis have shown that biosynthesis genes for some antibiotics are clustered into polycistronic transcription units and are under the control of global regulatory systems that govern the expression of genes that are induced when Bacillus cells enter stationary phase of growth. Future experiments involving the molecular dissection of peptide antibiotic biosynthesis genes in Bacillus will be attempted in hopes of further examining the mechanism and regulation of antibiotic production.

Original language	English (US)
Pages (from-to)	223-240
Number of pages	18
Journal	Critical reviews in biotechnology
Volume	10
Issue number	3
DOIs	https://doi.org/10.3109/07388559009038209
State	Published - 1990
Externally published	Yes

Funding

We would like to thank David A. Hopwood for his valuable information of antibiotic biosynthesis genes in Streptomyces. Thanks are also due to David Dubnau, Mohamed A. Marahiel, and Hiroaki Ishihara for their unpublished results. The work in the authors' laboratory was supported by Public Health Service grant GM39479-01 from the National Institutes of Health (to P. Z.) and grant HR8-3342 from the Oklahoma Center for the Advancement of Science and Technology (to P. Z.).

Funders	Funder number
Author National Institutes of Health National Institutes of Health National Institutes of Health National Institutes of Health The Bev Hartig Huntington's Disease Foundation National Institutes of Health	HR8-3342
National Institute of General Medical Sciences	R29GM039479
U.S. Public Health Service	GM39479-01
Oklahoma Center for the Advancement of Science and Technology

ASJC Scopus subject areas

Biotechnology
Applied Microbiology and Biotechnology

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title = "Molecular biology of antibiotic production in bacillus",

abstract = "Several species of the genus Bacillus produce peptide antibiotics which are synthesized either through a ribosomal or non-ribosomal mechanism. The antibiotics gramicidin, tyrocidine, and bacitracin are synthesized nonribosomally by the multienzyme thiotemplate mechanism. Surfactin and mycobacillin are also synthesized nonribosomally but by a mechanism that, apparently, is distinct from that of the multienzyme thiotemplate. Other antibiotics such as subtilin are gene encoded and are ribosomally synthesized. Molecular genetic and DNA sequence analysis have shown that biosynthesis genes for some antibiotics are clustered into polycistronic transcription units and are under the control of global regulatory systems that govern the expression of genes that are induced when Bacillus cells enter stationary phase of growth. Future experiments involving the molecular dissection of peptide antibiotic biosynthesis genes in Bacillus will be attempted in hopes of further examining the mechanism and regulation of antibiotic production.",

author = "Nakano, \{Michiko M.\} and Peter Zuber",

note = "Funding Information: We would like to thank David A. Hopwood for his valuable information of antibiotic biosynthesis genes in Streptomyces. Thanks are also due to David Dubnau, Mohamed A. Marahiel, and Hiroaki Ishihara for their unpublished results. The work in the authors' laboratory was supported by Public Health Service grant GM39479-01 from the National Institutes of Health (to P. Z.) and grant HR8-3342 from the Oklahoma Center for the Advancement of Science and Technology (to P. Z.).",

year = "1990",

doi = "10.3109/07388559009038209",

language = "English (US)",

volume = "10",

pages = "223--240",

journal = "Critical reviews in biotechnology",

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T1 - Molecular biology of antibiotic production in bacillus

AU - Nakano, Michiko M.

AU - Zuber, Peter

N1 - Funding Information: We would like to thank David A. Hopwood for his valuable information of antibiotic biosynthesis genes in Streptomyces. Thanks are also due to David Dubnau, Mohamed A. Marahiel, and Hiroaki Ishihara for their unpublished results. The work in the authors' laboratory was supported by Public Health Service grant GM39479-01 from the National Institutes of Health (to P. Z.) and grant HR8-3342 from the Oklahoma Center for the Advancement of Science and Technology (to P. Z.).

PY - 1990

Y1 - 1990

N2 - Several species of the genus Bacillus produce peptide antibiotics which are synthesized either through a ribosomal or non-ribosomal mechanism. The antibiotics gramicidin, tyrocidine, and bacitracin are synthesized nonribosomally by the multienzyme thiotemplate mechanism. Surfactin and mycobacillin are also synthesized nonribosomally but by a mechanism that, apparently, is distinct from that of the multienzyme thiotemplate. Other antibiotics such as subtilin are gene encoded and are ribosomally synthesized. Molecular genetic and DNA sequence analysis have shown that biosynthesis genes for some antibiotics are clustered into polycistronic transcription units and are under the control of global regulatory systems that govern the expression of genes that are induced when Bacillus cells enter stationary phase of growth. Future experiments involving the molecular dissection of peptide antibiotic biosynthesis genes in Bacillus will be attempted in hopes of further examining the mechanism and regulation of antibiotic production.

AB - Several species of the genus Bacillus produce peptide antibiotics which are synthesized either through a ribosomal or non-ribosomal mechanism. The antibiotics gramicidin, tyrocidine, and bacitracin are synthesized nonribosomally by the multienzyme thiotemplate mechanism. Surfactin and mycobacillin are also synthesized nonribosomally but by a mechanism that, apparently, is distinct from that of the multienzyme thiotemplate. Other antibiotics such as subtilin are gene encoded and are ribosomally synthesized. Molecular genetic and DNA sequence analysis have shown that biosynthesis genes for some antibiotics are clustered into polycistronic transcription units and are under the control of global regulatory systems that govern the expression of genes that are induced when Bacillus cells enter stationary phase of growth. Future experiments involving the molecular dissection of peptide antibiotic biosynthesis genes in Bacillus will be attempted in hopes of further examining the mechanism and regulation of antibiotic production.

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Molecular biology of antibiotic production in bacillus

Abstract

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ASJC Scopus subject areas

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