Molecular characterization of two genes encoding members of the glucose transporter superfamily in the parasitic protozoan Leishmania donovani

Chris K. Langford; Shawn A. Ewbank; Sheri S. Hanson; Buddy Ullman; Scott M. Landfear

doi:10.1016/0166-6851(92)90126-5

Molecular characterization of two genes encoding members of the glucose transporter superfamily in the parasitic protozoan Leishmania donovani

Chris K. Langford
, Shawn A. Ewbank
, Sheri S. Hanson
, Buddy Ullman
, Scott M. Landfear

Molecular Microbiology and Immunology

Research output: Contribution to journal › Article › peer-review

42 Scopus citations

Abstract

The polymerase chain reaction was used to clone two genes from Leishmania donovani, each of which encodes a member of a superfamily of membrane transporters which include the mammalian facilitative glucose transporters. One of these transporters, designated D2, is similar in sequence and overall structural features to a previously cloned Leishmania transporter Pro-1. Both D2 and Pro-1 are developmentally regulated genes which are expressed primarily in the insect stage of the parasite life cycle. In contrast, the second novel transporter, D1, is structurally quite different from different from either D2 or Pro-1, and its expression is not regulated during the parasite life cycle. All three genes are located on different chromosomes in L. donovani.

Original language	English (US)
Pages (from-to)	51-64
Number of pages	14
Journal	Molecular and Biochemical Parasitology
Volume	55
Issue number	1-2
DOIs	https://doi.org/10.1016/0166-6851(92)90126-5
State	Published - Oct 1992

Funding

This research was supported by grants AI25920 (S.M.L.) and AI23682 (B.U.) from the National Institutes of Health and by a grant from the UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases (TDR) to S.M.L. S.M.L. also acknowledges a New Investigator Award in Molecular Parasitology from the Burroughs Wellcome Fund. We would like to thank Tom Allen for generous technical help with the PCR procedures used to map the 5' end of the D2 mRNA.

Funders	Funder number
UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases
Author National Institutes of Health National Institutes of Health National Institutes of Health National Institutes of Health The Bev Hartig Huntington's Disease Foundation National Institutes of Health
National Institute of Allergy and Infectious Diseases	R01AI023682
Burroughs Wellcome Fund

Keywords

Developmental regulation
Glucose transporter superfamily
Leishmania
Polymerase chain reaction

ASJC Scopus subject areas

Parasitology
Molecular Biology

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10.1016/0166-6851(92)90126-5

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title = "Molecular characterization of two genes encoding members of the glucose transporter superfamily in the parasitic protozoan Leishmania donovani",

abstract = "The polymerase chain reaction was used to clone two genes from Leishmania donovani, each of which encodes a member of a superfamily of membrane transporters which include the mammalian facilitative glucose transporters. One of these transporters, designated D2, is similar in sequence and overall structural features to a previously cloned Leishmania transporter Pro-1. Both D2 and Pro-1 are developmentally regulated genes which are expressed primarily in the insect stage of the parasite life cycle. In contrast, the second novel transporter, D1, is structurally quite different from different from either D2 or Pro-1, and its expression is not regulated during the parasite life cycle. All three genes are located on different chromosomes in L. donovani.",

keywords = "Developmental regulation, Glucose transporter superfamily, Leishmania, Polymerase chain reaction",

author = "Langford, \{Chris K.\} and Ewbank, \{Shawn A.\} and Hanson, \{Sheri S.\} and Buddy Ullman and Landfear, \{Scott M.\}",

note = "Funding Information: This research was supported by grants AI25920 (S.M.L.) and AI23682 (B.U.) from the National Institutes of Health and by a grant from the UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases (TDR) to S.M.L. S.M.L. also acknowledges a New Investigator Award in Molecular Parasitology from the Burroughs Wellcome Fund. We would like to thank Tom Allen for generous technical help with the PCR procedures used to map the 5' end of the D2 mRNA.",

year = "1992",

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doi = "10.1016/0166-6851(92)90126-5",

language = "English (US)",

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AU - Ewbank, Shawn A.

AU - Hanson, Sheri S.

AU - Ullman, Buddy

AU - Landfear, Scott M.

N1 - Funding Information: This research was supported by grants AI25920 (S.M.L.) and AI23682 (B.U.) from the National Institutes of Health and by a grant from the UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases (TDR) to S.M.L. S.M.L. also acknowledges a New Investigator Award in Molecular Parasitology from the Burroughs Wellcome Fund. We would like to thank Tom Allen for generous technical help with the PCR procedures used to map the 5' end of the D2 mRNA.

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N2 - The polymerase chain reaction was used to clone two genes from Leishmania donovani, each of which encodes a member of a superfamily of membrane transporters which include the mammalian facilitative glucose transporters. One of these transporters, designated D2, is similar in sequence and overall structural features to a previously cloned Leishmania transporter Pro-1. Both D2 and Pro-1 are developmentally regulated genes which are expressed primarily in the insect stage of the parasite life cycle. In contrast, the second novel transporter, D1, is structurally quite different from different from either D2 or Pro-1, and its expression is not regulated during the parasite life cycle. All three genes are located on different chromosomes in L. donovani.

AB - The polymerase chain reaction was used to clone two genes from Leishmania donovani, each of which encodes a member of a superfamily of membrane transporters which include the mammalian facilitative glucose transporters. One of these transporters, designated D2, is similar in sequence and overall structural features to a previously cloned Leishmania transporter Pro-1. Both D2 and Pro-1 are developmentally regulated genes which are expressed primarily in the insect stage of the parasite life cycle. In contrast, the second novel transporter, D1, is structurally quite different from different from either D2 or Pro-1, and its expression is not regulated during the parasite life cycle. All three genes are located on different chromosomes in L. donovani.

KW - Developmental regulation

KW - Glucose transporter superfamily

KW - Leishmania

KW - Polymerase chain reaction

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Molecular characterization of two genes encoding members of the glucose transporter superfamily in the parasitic protozoan Leishmania donovani

Abstract

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Keywords

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