Molecular identity, synaptic localization, and physiology of calcium channels in retinal bipolar cells

Amy Berntson; W. Rowland Taylor; Catherine W. Morgans

doi:10.1002/jnr.10459

Molecular identity, synaptic localization, and physiology of calcium channels in retinal bipolar cells

Amy Berntson, W. Rowland Taylor, Catherine W. Morgans

Research output: Contribution to journal › Article › peer-review

71 Scopus citations

Abstract

Bipolar cells convey information through the retina via graded changes in their membrane potential and modulate transmitter release through the influx of calcium via L-type calcium channels. However, the molecular identity of the α₁ subunit has not been confirmed. We report the presence of the newly cloned α_1F subunit in mouse bipolar cell synaptic terminals. The α_1F subunits are localized to hot spots, possibly corresponding to active zones. We also report the physiological properties of two calcium currents present in mouse bipolar cells, a low-voltage-activated L-type current and a low-voltage-activated T-type calcium current. The physiological properties of the T-type current suggest that it is completely inactivated under physiological conditions. The L-type current may be mediated by the α_1F subunit, and influx of calcium through the α_1F channel may control neurotransmitter release from the bipolar cell terminal.

Original language	English (US)
Pages (from-to)	146-151
Number of pages	6
Journal	Journal of Neuroscience Research
Volume	71
Issue number	1
DOIs	https://doi.org/10.1002/jnr.10459
State	Published - Jan 1 2003

Keywords

Alpha 1F
Electrophysiology
Immunohistochemistry
Mouse
Patch-clamp

ASJC Scopus subject areas

Cellular and Molecular Neuroscience

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10.1002/jnr.10459

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title = "Molecular identity, synaptic localization, and physiology of calcium channels in retinal bipolar cells",

abstract = "Bipolar cells convey information through the retina via graded changes in their membrane potential and modulate transmitter release through the influx of calcium via L-type calcium channels. However, the molecular identity of the α1 subunit has not been confirmed. We report the presence of the newly cloned α1F subunit in mouse bipolar cell synaptic terminals. The α1F subunits are localized to hot spots, possibly corresponding to active zones. We also report the physiological properties of two calcium currents present in mouse bipolar cells, a low-voltage-activated L-type current and a low-voltage-activated T-type calcium current. The physiological properties of the T-type current suggest that it is completely inactivated under physiological conditions. The L-type current may be mediated by the α1F subunit, and influx of calcium through the α1F channel may control neurotransmitter release from the bipolar cell terminal.",

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AU - Taylor, W. Rowland

AU - Morgans, Catherine W.

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AB - Bipolar cells convey information through the retina via graded changes in their membrane potential and modulate transmitter release through the influx of calcium via L-type calcium channels. However, the molecular identity of the α1 subunit has not been confirmed. We report the presence of the newly cloned α1F subunit in mouse bipolar cell synaptic terminals. The α1F subunits are localized to hot spots, possibly corresponding to active zones. We also report the physiological properties of two calcium currents present in mouse bipolar cells, a low-voltage-activated L-type current and a low-voltage-activated T-type calcium current. The physiological properties of the T-type current suggest that it is completely inactivated under physiological conditions. The L-type current may be mediated by the α1F subunit, and influx of calcium through the α1F channel may control neurotransmitter release from the bipolar cell terminal.

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KW - Electrophysiology

KW - Immunohistochemistry

KW - Mouse

KW - Patch-clamp

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Molecular identity, synaptic localization, and physiology of calcium channels in retinal bipolar cells

Abstract

Keywords

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