TY - JOUR
T1 - MR of carcinoma-specific monoclonal antibody conjugated to monocrystalline iron oxide nanoparticles
T2 - The potential for noninvasive diagnosis
AU - Remsen, Laura G.
AU - McCormick, Christopher I.
AU - Roman-Goldstein, Simon
AU - Nilaver, Gajanan
AU - Weissleder, Ralph
AU - Bogdanov, Alexei
AU - Hellström, Karl E.
AU - Hellström, Ingegerd
AU - Kroll, Robert A.
AU - Neuwelt, Edward A.
PY - 1996
Y1 - 1996
N2 - PURPOSE: To determine if tumor-specific monoclonal antibodies conjugated to superparamagnetic monocrystalline iron oxide nanoparticles can be used to yield specific diagnoses with the use of MR imaging. METHODS: Monoclonal antibodies conjugated to monocrystalline iron oxide nanoparticles were given to nude rats with intracranial tumors either by intravenous injection, intraarterial injection with osmotic blood-brain barrier disruption, or direct intratumoral inoculation. Either L6, a tumor-specific antibody, or P- 1.17, a control isotype-matched antibody, was used. Coronal T1-weighted, T2- weighted, and spoiled gradient-recalled acquisition in the steady state images were obtained before, 30 minutes after, 6 hours after, and 24 hours after injection. RESULTS: intravenous injection of greater than 2 mg of the tumor-specific antibody showed a specific pattern of enhancement of the tumors with the largest concentration of antibody in the area with the greatest density of tumor cells. The control antibody showed nonspecific changes. After intraarterial injection with barrier disruption to increase delivery globally or direct inoculation to increase delivery focally, no specific enhancement pattern was seen. CONCLUSION: Monoclonal antibodies conjugated with monocrystalline iron oxide particles may provide a method to obtain specific diagnoses with the use of MR imaging.
AB - PURPOSE: To determine if tumor-specific monoclonal antibodies conjugated to superparamagnetic monocrystalline iron oxide nanoparticles can be used to yield specific diagnoses with the use of MR imaging. METHODS: Monoclonal antibodies conjugated to monocrystalline iron oxide nanoparticles were given to nude rats with intracranial tumors either by intravenous injection, intraarterial injection with osmotic blood-brain barrier disruption, or direct intratumoral inoculation. Either L6, a tumor-specific antibody, or P- 1.17, a control isotype-matched antibody, was used. Coronal T1-weighted, T2- weighted, and spoiled gradient-recalled acquisition in the steady state images were obtained before, 30 minutes after, 6 hours after, and 24 hours after injection. RESULTS: intravenous injection of greater than 2 mg of the tumor-specific antibody showed a specific pattern of enhancement of the tumors with the largest concentration of antibody in the area with the greatest density of tumor cells. The control antibody showed nonspecific changes. After intraarterial injection with barrier disruption to increase delivery globally or direct inoculation to increase delivery focally, no specific enhancement pattern was seen. CONCLUSION: Monoclonal antibodies conjugated with monocrystalline iron oxide particles may provide a method to obtain specific diagnoses with the use of MR imaging.
KW - Animal studies
KW - Antigens and antibodies
KW - Brain neoplasms, magnetic resonance
KW - Carcinoma
UR - http://www.scopus.com/inward/record.url?scp=9244257373&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=9244257373&partnerID=8YFLogxK
M3 - Article
C2 - 8881233
AN - SCOPUS:9244257373
SN - 0195-6108
VL - 17
SP - 411
EP - 418
JO - American Journal of Neuroradiology
JF - American Journal of Neuroradiology
IS - 3
ER -