Mucus secretions from a conditionally reprogrammed primary endocervical cell culture

Daye Park; Ashok Reddy; Phillip A. Wilmarth; Jeffrey Jensen; Leo Han

doi:10.1016/j.xfss.2022.03.003

Mucus secretions from a conditionally reprogrammed primary endocervical cell culture

Daye Park
, Ashok Reddy
, Phillip A. Wilmarth
, Jeffrey Jensen
, Leo Han

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Objective: To determine if the secretions collected from a conditionally reprogrammed primary endocervical cell culture are suitable surrogates for mucus studies. Design: Experimental. Setting: University research center. Animal(s): Female rhesus macaque (n = 2). Intervention(s): None. Main Outcome Measure(s): Quantitative proteomic analysis using tandem mass tag mass spectrometry liquid chromatography/tandem mass spectrometry. Result(s): We identified 3,047 proteins, common proteins present in both primary endocervical cell cultures and the mucus of rhesus macaques. We found a 71% overlap in the top 500 most prevalent proteins in the samples. Cell culture secretions contained many essential mucus proteins, including MUC5B, the primary mucin of the endocervix. Conclusion(s): Similarities in secreted proteins suggest that conditionally reprogrammed primary endocervical cells could be used to study mucus secretion in vitro.

Original language	English (US)
Pages (from-to)	159-165
Number of pages	7
Journal	F and S Science
Volume	3
Issue number	2
DOIs	https://doi.org/10.1016/j.xfss.2022.03.003
State	Published - May 1 2022

Funding

D.P. has nothing to disclose. A.P.R. has nothing to disclose. P.A.W. has nothing to disclose. J.T.J. has nothing to disclose. L.H. reports grants from Reproductive Scientist Development Program–NICHD grant K12 HD00849, The March of Dimes Foundation, American Society for Reproductive Medicine, American Board of Obstetrics and Gynecology, OHSU School of Medicine, Medical Foundation of Oregon and Oregon National Primate Research Center (core grant #P51OD011092), and NIH (grants P30EY010572, P30CA069533, and S10OD012246) for the submitted work. This research received support through the grant K12 HD000849, awarded to the Reproductive Scientist Development Program (RSDP) by the National Institute of Child Health and Human Development. In addition, this work received funding from the March of Dimes Foundation , American Society for Reproductive Medicine , the American Board of Obstetrics and Gynecology as part of the RSDP , and the Oregon Health & Science University School of Medicine , Medical Foundation of Oregon , and Oregon National Primate Research Center core grant number P51 OD011092. Mass spectrometry was done at the Oregon Health & Science University Proteomics Shared Resource with partial support from National Institute of Health grants P30EY010572, P30CA069533, and S10OD012246.

Funders	Funder number
American Board of Obstetrics and Gynecology
OHSU School of Medicine, Medical Foundation of Oregon
Author National Institutes of Health National Institutes of Health National Institutes of Health National Institutes of Health The Bev Hartig Huntington's Disease Foundation National Institutes of Health	P30CA069533, P30EY010572, S10OD012246
NIH Office of the Director	P51OD011092
The National Institute of Child Health and Human Development
March of Dimes Foundation
American Society for Reproductive Medicine
Oregon State University/Oregon Health and Science University
Oregon National Primate Research Center	P51 OD011092, 51OD011092
Eunice Kennedy Shriver National Institute of Child Health and Human Development, National Institutes of Health	K12 HD00849

Keywords

Endocervix
mucus
primate
proteome

ASJC Scopus subject areas

Reproductive Medicine
Embryology

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10.1016/j.xfss.2022.03.003

Cite this

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title = "Mucus secretions from a conditionally reprogrammed primary endocervical cell culture",

abstract = "Objective: To determine if the secretions collected from a conditionally reprogrammed primary endocervical cell culture are suitable surrogates for mucus studies. Design: Experimental. Setting: University research center. Animal(s): Female rhesus macaque (n = 2). Intervention(s): None. Main Outcome Measure(s): Quantitative proteomic analysis using tandem mass tag mass spectrometry liquid chromatography/tandem mass spectrometry. Result(s): We identified 3,047 proteins, common proteins present in both primary endocervical cell cultures and the mucus of rhesus macaques. We found a 71\% overlap in the top 500 most prevalent proteins in the samples. Cell culture secretions contained many essential mucus proteins, including MUC5B, the primary mucin of the endocervix. Conclusion(s): Similarities in secreted proteins suggest that conditionally reprogrammed primary endocervical cells could be used to study mucus secretion in vitro.",

keywords = "Endocervix, mucus, primate, proteome",

author = "Daye Park and Ashok Reddy and Wilmarth, \{Phillip A.\} and Jeffrey Jensen and Leo Han",

note = "Publisher Copyright: {\textcopyright} 2022 American Society for Reproductive Medicine",

year = "2022",

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doi = "10.1016/j.xfss.2022.03.003",

language = "English (US)",

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T1 - Mucus secretions from a conditionally reprogrammed primary endocervical cell culture

AU - Park, Daye

AU - Reddy, Ashok

AU - Wilmarth, Phillip A.

AU - Jensen, Jeffrey

AU - Han, Leo

PY - 2022/5/1

Y1 - 2022/5/1

N2 - Objective: To determine if the secretions collected from a conditionally reprogrammed primary endocervical cell culture are suitable surrogates for mucus studies. Design: Experimental. Setting: University research center. Animal(s): Female rhesus macaque (n = 2). Intervention(s): None. Main Outcome Measure(s): Quantitative proteomic analysis using tandem mass tag mass spectrometry liquid chromatography/tandem mass spectrometry. Result(s): We identified 3,047 proteins, common proteins present in both primary endocervical cell cultures and the mucus of rhesus macaques. We found a 71% overlap in the top 500 most prevalent proteins in the samples. Cell culture secretions contained many essential mucus proteins, including MUC5B, the primary mucin of the endocervix. Conclusion(s): Similarities in secreted proteins suggest that conditionally reprogrammed primary endocervical cells could be used to study mucus secretion in vitro.

AB - Objective: To determine if the secretions collected from a conditionally reprogrammed primary endocervical cell culture are suitable surrogates for mucus studies. Design: Experimental. Setting: University research center. Animal(s): Female rhesus macaque (n = 2). Intervention(s): None. Main Outcome Measure(s): Quantitative proteomic analysis using tandem mass tag mass spectrometry liquid chromatography/tandem mass spectrometry. Result(s): We identified 3,047 proteins, common proteins present in both primary endocervical cell cultures and the mucus of rhesus macaques. We found a 71% overlap in the top 500 most prevalent proteins in the samples. Cell culture secretions contained many essential mucus proteins, including MUC5B, the primary mucin of the endocervix. Conclusion(s): Similarities in secreted proteins suggest that conditionally reprogrammed primary endocervical cells could be used to study mucus secretion in vitro.

KW - Endocervix

KW - mucus

KW - primate

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Mucus secretions from a conditionally reprogrammed primary endocervical cell culture

Abstract

Funding

Keywords

ASJC Scopus subject areas

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