@inbook{61dcd8f613084c48a1817fb74d0fab17,
title = "Multiplex imaging of polymicrobial communities—murine models to study oral microbiome interactions",
abstract = "Similar to other mucosal surfaces of the body, the oral cavity hosts a diverse microbial flora that live in polymicrobial biofilm communities. It is the ecology of these communities that are the primary determinants of oral health (symbiosis) or disease (dysbiosis). As such, both symbiosis and dysbiosis are inherently polymicrobial phenomena. In an effort to facilitate studies of polymicrobial communities within rodent models, we developed a suite of synthetic luciferases suitable for multiplexed in situ analyses of microbial ecology and specific gene expression. Using this approach, it is feasible to noninvasively measure multiple luciferase signals in vivo with both spatial and temporal resolution. In the following chapter, we describe the relevant details and protocols used to establish a biophotonic imaging platform for the study of experimental polymicrobial oral biofilms and abscesses in mice. The protocols described here are specifically tailored for use with oral streptococci, but the general strategies are adaptable for a wide range of polymicrobial infection studies using other species.",
keywords = "Abscess, Animal model, Biofilm, Bioluminescent imaging, Biophotonic, Dental plaque, Luciferase, Microbiome, Polymicrobial infection, Streptococcus",
author = "Jens Kreth and Abdelrahman, {Yasser M.} and Justin Merritt",
note = "Funding Information: This work was supported by an NIH/NIDCR grant R35DE0282252 to J.M. and NIH/NIDCR grants R01DE021726 and R56DE021726 to J.K. Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2020.",
year = "2020",
doi = "10.1007/978-1-4939-9940-8_8",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "107--126",
booktitle = "Methods in Molecular Biology",
}