TY - JOUR
T1 - Multiscale cardiac imaging spanning the whole heart and its internal cellular architecture in a small animal model
AU - Rykiel, Graham
AU - López, Claudia S.
AU - Riesterer, Jessica L.
AU - Fries, Ian
AU - Deosthali, Sanika
AU - Courchaine, Katherine
AU - Maloyan, Alina
AU - Thornburg, Kent
AU - Rugonyi, Sandra
N1 - Funding Information:
This work has been funded by a grant from US National Institutes of Health, NIH R01 HL094570 (SR); the OHSU University Shared Resource pilot funding program (SR); the OHSU School of Medicine Faculty Innovation Fund (SR). We thank Kevin Loftis for graciously sharing his time and knowledge of Amira. We also thank Melissa Williams for her help in optimizing our sample preparation and Renovo Neural, Inc, especially Emily Benson and Grahame Kidd, for sharing their SBF-SEM protocol. Electron microscopy was performed at the OHSU Multiscale Microscopy Core (MMC) with technical support from the OHSU Center for Spatial Systems Biomedicine (OCSSB). An extended abstract (two pages) of this work was published and presented at Microscopy & Microanalysis 2019 Meeting in Portland, OR, https://www.cambridge.org/core/journals/microscopy-and-microanalysis/article/multiscale-cardiac-imaging-from-whole-heart-images-to-cardiac-ultrastruc-ture/7053ED929882C2E43B2ED85FD6D78BEC.
Funding Information:
This work has been funded by a grant from US National Institutes of Health, NIH R01 HL094570 (SR); the OHSU University Shared Resource pilot funding program (SR); the OHSU School of Medicine Faculty Innovation Fund (SR). We thank Kevin Loftis for graciously sharing his time and knowledge of Amira. We also thank Melissa Williams for her help in optimizing our sample preparation and Renovo Neural, Inc, especially Emily Benson and Grahame Kidd, for sharing their SBF-SEM protocol. Electron microscopy was performed at the OHSU Multiscale Microscopy Core (MMC) with technical support from the OHSU Center for Spatial Systems Biomedicine (OCSSB).
Publisher Copyright:
© Rykiel et al.
PY - 2020/10
Y1 - 2020/10
N2 - Cardiac pumping depends on the morphological structure of the heart, but also on its subcellular (ultrastructural) architecture, which enables cardiac contraction. In cases of congenital heart defects, localized ultrastructural disruptions that increase the risk of heart failure are only starting to be discovered. This is in part due to a lack of technologies that can image the three-dimensional (3D) heart structure, to assess malformations; and its ultrastructure, to assess organelle disruptions. We present here a multiscale, correlative imaging procedure that achieves high-resolution images of the whole heart, using 3D micro-computed tomography (micro-CT); and its ultrastructure, using 3D scanning electron microscopy (SEM). In a small animal model (chicken embryo), we achieved uniform fixation and staining of the whole heart, without losing ultrastructural preservation on the same sample, enabling correlative multiscale imaging. Our approach enables multiscale studies in models of congenital heart disease and beyond.
AB - Cardiac pumping depends on the morphological structure of the heart, but also on its subcellular (ultrastructural) architecture, which enables cardiac contraction. In cases of congenital heart defects, localized ultrastructural disruptions that increase the risk of heart failure are only starting to be discovered. This is in part due to a lack of technologies that can image the three-dimensional (3D) heart structure, to assess malformations; and its ultrastructure, to assess organelle disruptions. We present here a multiscale, correlative imaging procedure that achieves high-resolution images of the whole heart, using 3D micro-computed tomography (micro-CT); and its ultrastructure, using 3D scanning electron microscopy (SEM). In a small animal model (chicken embryo), we achieved uniform fixation and staining of the whole heart, without losing ultrastructural preservation on the same sample, enabling correlative multiscale imaging. Our approach enables multiscale studies in models of congenital heart disease and beyond.
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U2 - 10.7554/eLife.58138
DO - 10.7554/eLife.58138
M3 - Article
C2 - 33078706
AN - SCOPUS:85094982945
SN - 2050-084X
VL - 9
SP - 1
EP - 26
JO - eLife
JF - eLife
M1 - e58138
ER -