Multiscale light-sheet for rapid imaging of cardiopulmonary system

Yichen Ding; Jianguo Ma; Adam D. Langenbacher; Kyung In Baek; Juhyun Lee; Chih Chiang Chang; Jeffrey J. Hsu; Rajan P. Kulkarni; John Belperio; Wei Shi; Sara Ranjbarvaziri; Reza Ardehali; Yin Tintut; Linda L. Demer; Jau Nian Chen; Peng Fei; René R.Sevag Packard; Tzung K. Hsiai

doi:10.1172/jci.insight.121396

Multiscale light-sheet for rapid imaging of cardiopulmonary system

Yichen Ding, Jianguo Ma, Adam D. Langenbacher, Kyung In Baek, Juhyun Lee, Chih Chiang Chang, Jeffrey J. Hsu, Rajan P. Kulkarni, John Belperio, Wei Shi, Sara Ranjbarvaziri, Reza Ardehali, Yin Tintut, Linda L. Demer, Jau Nian Chen, Peng Fei, René R.Sevag Packard, Tzung K. Hsiai

Research output: Contribution to journal › Review article › peer-review

30 Scopus citations

Abstract

The ability to image tissue morphogenesis in real-time and in 3-dimensions (3-D) remains an optical challenge. The advent of light-sheet fluorescence microscopy (LSFM) has advanced developmental biology and tissue regeneration research. In this review, we introduce a LSFM system in which the illumination lens reshapes a thin light-sheet to rapidly scan across a sample of interest while the detection lens orthogonally collects the imaging data. This multiscale strategy provides deep-tissue penetration, high-spatiotemporal resolution, and minimal photobleaching and phototoxicity, allowing in vivo visualization of a variety of tissues and processes, ranging from developing hearts in live zebrafish embryos to ex vivo interrogation of the microarchitecture of optically cleared neonatal hearts. Here, we highlight multiple applications of LSFM and discuss several studies that have allowed better characterization of developmental and pathological processes in multiple models and tissues. These findings demonstrate the capacity of multiscale light-sheet imaging to uncover cardiovascular developmental and regenerative phenomena.

Original language	English (US)
Journal	JCI Insight
Volume	3
Issue number	16
DOIs	https://doi.org/10.1172/jci.insight.121396
State	Published - Aug 23 2018
Externally published	Yes

ASJC Scopus subject areas

General Medicine

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Ding, Y., Ma, J., Langenbacher, A. D., Baek, K. I., Lee, J., Chang, C. C., Hsu, J. J., Kulkarni, R. P., Belperio, J., Shi, W., Ranjbarvaziri, S., Ardehali, R., Tintut, Y., Demer, L. L., Chen, J. N., Fei, P., Packard, R. R. S., & Hsiai, T. K. (2018). Multiscale light-sheet for rapid imaging of cardiopulmonary system. JCI Insight, 3(16). https://doi.org/10.1172/jci.insight.121396

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abstract = "The ability to image tissue morphogenesis in real-time and in 3-dimensions (3-D) remains an optical challenge. The advent of light-sheet fluorescence microscopy (LSFM) has advanced developmental biology and tissue regeneration research. In this review, we introduce a LSFM system in which the illumination lens reshapes a thin light-sheet to rapidly scan across a sample of interest while the detection lens orthogonally collects the imaging data. This multiscale strategy provides deep-tissue penetration, high-spatiotemporal resolution, and minimal photobleaching and phototoxicity, allowing in vivo visualization of a variety of tissues and processes, ranging from developing hearts in live zebrafish embryos to ex vivo interrogation of the microarchitecture of optically cleared neonatal hearts. Here, we highlight multiple applications of LSFM and discuss several studies that have allowed better characterization of developmental and pathological processes in multiple models and tissues. These findings demonstrate the capacity of multiscale light-sheet imaging to uncover cardiovascular developmental and regenerative phenomena.",

author = "Yichen Ding and Jianguo Ma and Langenbacher, {Adam D.} and Baek, {Kyung In} and Juhyun Lee and Chang, {Chih Chiang} and Hsu, {Jeffrey J.} and Kulkarni, {Rajan P.} and John Belperio and Wei Shi and Sara Ranjbarvaziri and Reza Ardehali and Yin Tintut and Demer, {Linda L.} and Chen, {Jau Nian} and Peng Fei and Packard, {Ren{\'e} R.Sevag} and Hsiai, {Tzung K.}",

year = "2018",

month = aug,

day = "23",

doi = "10.1172/jci.insight.121396",

language = "English (US)",

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AU - Ding, Yichen

AU - Ma, Jianguo

AU - Langenbacher, Adam D.

AU - Baek, Kyung In

AU - Lee, Juhyun

AU - Chang, Chih Chiang

AU - Hsu, Jeffrey J.

AU - Kulkarni, Rajan P.

AU - Belperio, John

AU - Shi, Wei

AU - Ranjbarvaziri, Sara

AU - Ardehali, Reza

AU - Tintut, Yin

AU - Demer, Linda L.

AU - Chen, Jau Nian

AU - Fei, Peng

AU - Packard, René R.Sevag

AU - Hsiai, Tzung K.

PY - 2018/8/23

Y1 - 2018/8/23

N2 - The ability to image tissue morphogenesis in real-time and in 3-dimensions (3-D) remains an optical challenge. The advent of light-sheet fluorescence microscopy (LSFM) has advanced developmental biology and tissue regeneration research. In this review, we introduce a LSFM system in which the illumination lens reshapes a thin light-sheet to rapidly scan across a sample of interest while the detection lens orthogonally collects the imaging data. This multiscale strategy provides deep-tissue penetration, high-spatiotemporal resolution, and minimal photobleaching and phototoxicity, allowing in vivo visualization of a variety of tissues and processes, ranging from developing hearts in live zebrafish embryos to ex vivo interrogation of the microarchitecture of optically cleared neonatal hearts. Here, we highlight multiple applications of LSFM and discuss several studies that have allowed better characterization of developmental and pathological processes in multiple models and tissues. These findings demonstrate the capacity of multiscale light-sheet imaging to uncover cardiovascular developmental and regenerative phenomena.

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Multiscale light-sheet for rapid imaging of cardiopulmonary system

Abstract

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