TY - JOUR
T1 - Mutations associated with viral sequences isolated from mice persistently infected with MHV-JHM
AU - Fleming, J. O.
AU - Adami, C.
AU - Pooley, J.
AU - Glomb, J.
AU - Stecker, E.
AU - Fazal, F.
AU - Baker, S. C.
PY - 1995
Y1 - 1995
N2 - Mouse hepatitis virus JHM (JHMV or MHV-4) induces subacute and chronic demyelination in rodents and has been studied as a model of human demyelinating diseases, such as multiple sclerosis. However, despite intensive investigation, the state of JHMV during chronic disease is poorly understood. Using reverse transcription-polymerase chain reaction amplification (RT-PCR) to 'rescue' viral RNA, we have found that JHMV- specific sequences persist for at least 787 days after intracerebral inoculation of experimental mice. Analysis of persisting vital RNA reveals that it is extensively mutated, and we hypothesize that the mutations observed reflect adaptation of the vital quasispecies to low-level intracellular replication during chronic disease.
AB - Mouse hepatitis virus JHM (JHMV or MHV-4) induces subacute and chronic demyelination in rodents and has been studied as a model of human demyelinating diseases, such as multiple sclerosis. However, despite intensive investigation, the state of JHMV during chronic disease is poorly understood. Using reverse transcription-polymerase chain reaction amplification (RT-PCR) to 'rescue' viral RNA, we have found that JHMV- specific sequences persist for at least 787 days after intracerebral inoculation of experimental mice. Analysis of persisting vital RNA reveals that it is extensively mutated, and we hypothesize that the mutations observed reflect adaptation of the vital quasispecies to low-level intracellular replication during chronic disease.
UR - http://www.scopus.com/inward/record.url?scp=0028785127&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028785127&partnerID=8YFLogxK
U2 - 10.1007/978-1-4615-1899-0_94
DO - 10.1007/978-1-4615-1899-0_94
M3 - Article
C2 - 8830547
AN - SCOPUS:0028785127
SN - 0065-2598
VL - 380
SP - 591
EP - 595
JO - Advances in experimental medicine and biology
JF - Advances in experimental medicine and biology
ER -