TY - JOUR
T1 - Myosin 1c participates in B cell cytoskeleton rearrangements, is recruited to the immunologic synapse, and contributes to antigen presentation
AU - Maravillas-Montero, José L.
AU - Gillespie, Peter G.
AU - Patiño-López, Genaro
AU - Shaw, Stephen
AU - Santos-Argumedo, Leopoldo
PY - 2011/9/15
Y1 - 2011/9/15
N2 - Myosin 1c (Myo1c) is a member of the unconventional class I myosins of vertebrates, which directly link the plasma membrane with the microfilament cortical web. Although this molecular motor has been implicated in cell functions such as cytoskeleton organization, cell motility, nuclear transcription, and endocytosis, its role in hematopoietic cells is largely unknown. In this study, we show that Myo1c is abundantly expressed in murine B lymphocytes and is preferentially located at the plasma membrane, especially in peripheral processes such as microvilli.We observed that this motor concentrates at the growing membrane protrusions generated during B cell spreading and that it is actively recruited to the immune synapse. Interestingly, Myo1c was detected in lipid rafts of B cells and showed strong colocalization with MHC-II, particularly after cross-linking of these molecules. By transfection of a dominant negative formofMyo1c or specific siRNA,we also detected alterations in the spreading andAg-presenting ability of these cells. The data suggest that Myo1c is involved in the cytoskeleton dynamics and membrane protein anchoring or sorting in B lymphocytes.
AB - Myosin 1c (Myo1c) is a member of the unconventional class I myosins of vertebrates, which directly link the plasma membrane with the microfilament cortical web. Although this molecular motor has been implicated in cell functions such as cytoskeleton organization, cell motility, nuclear transcription, and endocytosis, its role in hematopoietic cells is largely unknown. In this study, we show that Myo1c is abundantly expressed in murine B lymphocytes and is preferentially located at the plasma membrane, especially in peripheral processes such as microvilli.We observed that this motor concentrates at the growing membrane protrusions generated during B cell spreading and that it is actively recruited to the immune synapse. Interestingly, Myo1c was detected in lipid rafts of B cells and showed strong colocalization with MHC-II, particularly after cross-linking of these molecules. By transfection of a dominant negative formofMyo1c or specific siRNA,we also detected alterations in the spreading andAg-presenting ability of these cells. The data suggest that Myo1c is involved in the cytoskeleton dynamics and membrane protein anchoring or sorting in B lymphocytes.
UR - http://www.scopus.com/inward/record.url?scp=80053078222&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=80053078222&partnerID=8YFLogxK
U2 - 10.4049/jimmunol.1004018
DO - 10.4049/jimmunol.1004018
M3 - Article
C2 - 21841128
AN - SCOPUS:80053078222
SN - 0022-1767
VL - 187
SP - 3053
EP - 3063
JO - Journal of Immunology
JF - Journal of Immunology
IS - 6
ER -