TY - JOUR
T1 - Neutrophils rescue gingival epithelial cells from bacterial-induced apoptosis
AU - Galicia, Johnah C.
AU - Benakanakere, Manjunatha R.
AU - Stathopoulou, Panagiota G.
AU - Kinane, Denis F.
PY - 2009/7/1
Y1 - 2009/7/1
N2 - In the pathogenesis of chronic inflammatory periodontal disease, neutrophils are recognized as a major cellular component from the histopathology of the periodontal lesion around teeth and from clinical cases where absence or dysfunction of neutrophils results in major periodontal destruction. Neutrophils are recruited in vast numbers into the gingival crevice during periodontal inflammation, attracted by microbial plaque chemoattractants and chemokines released following microbial perturbation of gingival epithelial cells. Porphyromonas gingivalis, a major periodontopathogen, triggers a vast array of cellular responses in gingival epithelial cells but also induces apoptosis. We demonstrate here that neutrophils, when combined in a P. gingivalis challenge assay of epithelial cells, prevent epithelial cell apoptosis by phagocytosing P. gingivalis and later undergoing apoptosis themselves. By removing P. gingivalis by phagocytosis, neutrophils also protect the host from the harmful effects of its microbial proteases, which degrade inflammatory cytokines and other host molecules.
AB - In the pathogenesis of chronic inflammatory periodontal disease, neutrophils are recognized as a major cellular component from the histopathology of the periodontal lesion around teeth and from clinical cases where absence or dysfunction of neutrophils results in major periodontal destruction. Neutrophils are recruited in vast numbers into the gingival crevice during periodontal inflammation, attracted by microbial plaque chemoattractants and chemokines released following microbial perturbation of gingival epithelial cells. Porphyromonas gingivalis, a major periodontopathogen, triggers a vast array of cellular responses in gingival epithelial cells but also induces apoptosis. We demonstrate here that neutrophils, when combined in a P. gingivalis challenge assay of epithelial cells, prevent epithelial cell apoptosis by phagocytosing P. gingivalis and later undergoing apoptosis themselves. By removing P. gingivalis by phagocytosis, neutrophils also protect the host from the harmful effects of its microbial proteases, which degrade inflammatory cytokines and other host molecules.
KW - Host defense
KW - Human
KW - Phagocytosis
UR - http://www.scopus.com/inward/record.url?scp=68849102241&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=68849102241&partnerID=8YFLogxK
U2 - 10.1189/jlb.0109003
DO - 10.1189/jlb.0109003
M3 - Article
C2 - 19389800
AN - SCOPUS:68849102241
SN - 0741-5400
VL - 86
SP - 181
EP - 186
JO - Journal of Leukocyte Biology
JF - Journal of Leukocyte Biology
IS - 1
ER -