NGF induction of the gene encoding the protease transin accompanies neuronal differentiation in PC12 cells

Cynthia M. Machida; Karin D. Rodland; Lynn Matrisian; Bruce E. Magun; Gary Ciment

doi:10.1016/0896-6273(89)90047-0

NGF induction of the gene encoding the protease transin accompanies neuronal differentiation in PC12 cells

Cynthia M. Machida
, Karin D. Rodland
, Lynn Matrisian
, Bruce E. Magun
, Gary Ciment

Cell Developmental and Cancer Biology

Research output: Contribution to journal › Article › peer-review

125 Scopus citations

Abstract

Various proteases have been found to be released by the growth cones of developing neurons in culture and have been hypothesized to play a role in the process of axon elongation. We report here that nerve growth factor (NGF) induced the gene encoding the metalloprotease transin in PC12 cells with a time course coincident with the initial appearance of neurites by these cells. Acidic and basic fibroblast growth factors also stimulated transin mRNA expression and neurite outgrowth, whereas various other agents had no effects on either of these phenomena. In contrast, dexamethasone was found to inhibit the induction of transin mRNA when added with, or following, NGF treatment. Finally, we show that sequences contained within 750 by of the 5′ untranscribed region of the transin gene confer responsiveness to NGF and dexamethasone.

Original language	English (US)
Pages (from-to)	1587-1596
Number of pages	10
Journal	Neuron
Volume	2
Issue number	6
DOIs	https://doi.org/10.1016/0896-6273(89)90047-0
State	Published - Jun 1989

Funding

The duthors wl,h to thank Dr. J.-P Julien for his gift of the NF-M plasmid, Dr. J Douglass for his gift of the cyclophilln plasmid, Dr. L. Baizer for his gift of NGF. Dr. R. Nishi for her helpful suggestions in the preliminary stages of this project, and Dr. K. Stocker for her critical reading of the manuscript. This work was supported by NIH grants to L. M (CA 48643). B. E. M. (CA 39360 and CA 47404). and G. C. (DE 07625 and NS23883) and by an NIH training grant stipend to C. M. M. (HDO 7133-10).

Funders	Funder number
Author National Institutes of Health National Institutes of Health National Institutes of Health National Institutes of Health The Bev Hartig Huntington's Disease Foundation National Institutes of Health	DE 07625, HDO 7133-10, NS23883
National Institute of Health-National Cancer Institute	R01CA048643

ASJC Scopus subject areas

General Neuroscience

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10.1016/0896-6273(89)90047-0

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@article{54b25601ca174faeb80a9af1d925bdb5,

title = "NGF induction of the gene encoding the protease transin accompanies neuronal differentiation in PC12 cells",

abstract = "Various proteases have been found to be released by the growth cones of developing neurons in culture and have been hypothesized to play a role in the process of axon elongation. We report here that nerve growth factor (NGF) induced the gene encoding the metalloprotease transin in PC12 cells with a time course coincident with the initial appearance of neurites by these cells. Acidic and basic fibroblast growth factors also stimulated transin mRNA expression and neurite outgrowth, whereas various other agents had no effects on either of these phenomena. In contrast, dexamethasone was found to inhibit the induction of transin mRNA when added with, or following, NGF treatment. Finally, we show that sequences contained within 750 by of the 5′ untranscribed region of the transin gene confer responsiveness to NGF and dexamethasone.",

author = "Machida, \{Cynthia M.\} and Rodland, \{Karin D.\} and Lynn Matrisian and Magun, \{Bruce E.\} and Gary Ciment",

note = "Funding Information: The duthors wl,h to thank Dr. J.-P Julien for his gift of the NF-M plasmid, Dr. J Douglass for his gift of the cyclophilln plasmid, Dr. L. Baizer for his gift of NGF. Dr. R. Nishi for her helpful suggestions in the preliminary stages of this project, and Dr. K. Stocker for her critical reading of the manuscript. This work was supported by NIH grants to L. M (CA 48643). B. E. M. (CA 39360 and CA 47404). and G. C. (DE 07625 and NS23883) and by an NIH training grant stipend to C. M. M. (HDO 7133-10).",

year = "1989",

month = jun,

doi = "10.1016/0896-6273(89)90047-0",

language = "English (US)",

volume = "2",

pages = "1587--1596",

journal = "Neuron",

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T1 - NGF induction of the gene encoding the protease transin accompanies neuronal differentiation in PC12 cells

AU - Machida, Cynthia M.

AU - Rodland, Karin D.

AU - Matrisian, Lynn

AU - Magun, Bruce E.

AU - Ciment, Gary

N1 - Funding Information: The duthors wl,h to thank Dr. J.-P Julien for his gift of the NF-M plasmid, Dr. J Douglass for his gift of the cyclophilln plasmid, Dr. L. Baizer for his gift of NGF. Dr. R. Nishi for her helpful suggestions in the preliminary stages of this project, and Dr. K. Stocker for her critical reading of the manuscript. This work was supported by NIH grants to L. M (CA 48643). B. E. M. (CA 39360 and CA 47404). and G. C. (DE 07625 and NS23883) and by an NIH training grant stipend to C. M. M. (HDO 7133-10).

PY - 1989/6

Y1 - 1989/6

N2 - Various proteases have been found to be released by the growth cones of developing neurons in culture and have been hypothesized to play a role in the process of axon elongation. We report here that nerve growth factor (NGF) induced the gene encoding the metalloprotease transin in PC12 cells with a time course coincident with the initial appearance of neurites by these cells. Acidic and basic fibroblast growth factors also stimulated transin mRNA expression and neurite outgrowth, whereas various other agents had no effects on either of these phenomena. In contrast, dexamethasone was found to inhibit the induction of transin mRNA when added with, or following, NGF treatment. Finally, we show that sequences contained within 750 by of the 5′ untranscribed region of the transin gene confer responsiveness to NGF and dexamethasone.

AB - Various proteases have been found to be released by the growth cones of developing neurons in culture and have been hypothesized to play a role in the process of axon elongation. We report here that nerve growth factor (NGF) induced the gene encoding the metalloprotease transin in PC12 cells with a time course coincident with the initial appearance of neurites by these cells. Acidic and basic fibroblast growth factors also stimulated transin mRNA expression and neurite outgrowth, whereas various other agents had no effects on either of these phenomena. In contrast, dexamethasone was found to inhibit the induction of transin mRNA when added with, or following, NGF treatment. Finally, we show that sequences contained within 750 by of the 5′ untranscribed region of the transin gene confer responsiveness to NGF and dexamethasone.

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NGF induction of the gene encoding the protease transin accompanies neuronal differentiation in PC12 cells

Abstract

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