Non-invasive cellular-resolution retinal imaging with two-photon excited fluorescence

Daniel J. Wahl; Myeong Jin Ju; Yifan Jian; Marinko V. Sarunic

doi:10.1364/BOE.10.004859

Non-invasive cellular-resolution retinal imaging with two-photon excited fluorescence

Daniel J. Wahl, Myeong Jin Ju, Yifan Jian, Marinko V. Sarunic

Ophthalmology

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Two-photon excited fluorescence (TPEF) imaging of the retina is a developing technique that provides non-invasive compound-specific measurements from the retina. In this report, we demonstrate high-resolution TPEF imaging of the mouse retina using sensorless adaptive optics (SAO) and optical coherence tomography (OCT). A single near-infrared light source was used for simultaneous multi-modal imaging with OCT and TPEF. The image-based SAO could be performed using the en face OCT or the TPEF for aberration correction. Our results demonstrate OCT and TPEF for angiography. Also, we demonstrate non-invasive cellular-resolution imaging of fluorescently labelled cells and the Retinal Pigment Epithelium (RPE) mosaic.

Original language	English (US)
Pages (from-to)	4859-4873
Number of pages	15
Journal	Biomedical Optics Express
Volume	10
Issue number	9
DOIs	https://doi.org/10.1364/BOE.10.004859
State	Published - Sep 1 2019

ASJC Scopus subject areas

Biotechnology
Atomic and Molecular Physics, and Optics

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title = "Non-invasive cellular-resolution retinal imaging with two-photon excited fluorescence",

abstract = "Two-photon excited fluorescence (TPEF) imaging of the retina is a developing technique that provides non-invasive compound-specific measurements from the retina. In this report, we demonstrate high-resolution TPEF imaging of the mouse retina using sensorless adaptive optics (SAO) and optical coherence tomography (OCT). A single near-infrared light source was used for simultaneous multi-modal imaging with OCT and TPEF. The image-based SAO could be performed using the en face OCT or the TPEF for aberration correction. Our results demonstrate OCT and TPEF for angiography. Also, we demonstrate non-invasive cellular-resolution imaging of fluorescently labelled cells and the Retinal Pigment Epithelium (RPE) mosaic.",

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AU - Ju, Myeong Jin

AU - Jian, Yifan

AU - Sarunic, Marinko V.

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AB - Two-photon excited fluorescence (TPEF) imaging of the retina is a developing technique that provides non-invasive compound-specific measurements from the retina. In this report, we demonstrate high-resolution TPEF imaging of the mouse retina using sensorless adaptive optics (SAO) and optical coherence tomography (OCT). A single near-infrared light source was used for simultaneous multi-modal imaging with OCT and TPEF. The image-based SAO could be performed using the en face OCT or the TPEF for aberration correction. Our results demonstrate OCT and TPEF for angiography. Also, we demonstrate non-invasive cellular-resolution imaging of fluorescently labelled cells and the Retinal Pigment Epithelium (RPE) mosaic.

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Non-invasive cellular-resolution retinal imaging with two-photon excited fluorescence

Abstract

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