Non-invasive cellular-resolution retinal imaging with two-photon excited fluorescence

Daniel J. Wahl, Myeong Jin Ju, Yifan Jian, Marinko V. Sarunic

Research output: Contribution to journalArticlepeer-review

13 Scopus citations


Two-photon excited fluorescence (TPEF) imaging of the retina is a developing technique that provides non-invasive compound-specific measurements from the retina. In this report, we demonstrate high-resolution TPEF imaging of the mouse retina using sensorless adaptive optics (SAO) and optical coherence tomography (OCT). A single near-infrared light source was used for simultaneous multi-modal imaging with OCT and TPEF. The image-based SAO could be performed using the en face OCT or the TPEF for aberration correction. Our results demonstrate OCT and TPEF for angiography. Also, we demonstrate non-invasive cellular-resolution imaging of fluorescently labelled cells and the Retinal Pigment Epithelium (RPE) mosaic.

Original languageEnglish (US)
Pages (from-to)4859-4873
Number of pages15
JournalBiomedical Optics Express
Issue number9
StatePublished - Sep 1 2019

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics


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