Nonaffinity Purification of Recombinant gp120 for Use in AIDS Vaccine Development

Carl J. Scandella; Jaleh Kilpatrick; William Lidster; Charles Parker; John P. Moore; Gregory K. Moore; Kimberly A. Mann; Peter Brown; Stephen Coates; Barbara Chapman; Frank R. Masiarz; Kathelyn S. Steimer; Nancy L. Haigwood

doi:10.1089/aid.1993.9.1233

Nonaffinity Purification of Recombinant gp120 for Use in AIDS Vaccine Development

Carl J. Scandella, Jaleh Kilpatrick, William Lidster, Charles Parker, John P. Moore, Gregory K. Moore, Kimberly A. Mann, Peter Brown, Stephen Coates, Barbara Chapman, Frank R. Masiarz, Kathelyn S. Steimer, Nancy L. Haigwood

Research output: Contribution to journal › Article › peer-review

43 Scopus citations

Abstract

The gene encoding the major envelope glycoprotein of the HIV-SF2 isolate was engineered for the secretion of recombinant gp120 (rgp120_SF2) from permanent Chinese hamster ovary (CHO) cell lines. Cellular production methods were scaled up and a method for purification of the secreted glycoprotein was devised. Mild purification conditions were selected in order to preserve the native structure of the protein. rgp120_SF2 exhibits a molecular weight of 120 kDa in reduced or nonreduced SDS gels; thus the polypeptide chain is intact. Deglycosylated rgp120_SF2 has the predicted molecular weight of the polypeptide backbone, 54 kDa. Gelfiltration HPLC in a nondenaturing buffer at neutral pH yields a molecular weight estimate of approximately 120 kDa. Purified rgp120 closely resembles authentic viral gp120 by several physical, chemical, and immunochemical tests. rgp120^SF2 reacts strongly with human HIV-positive sera, monoclonal antibodies reactive with HIV-SF2 and HIV-MN viral envelope, and a human virus-neutralizing monoclonal antibody that maps to a conserved discontinuous epitope on HIV-1 gp120. Purified rgp120_SF2 forms a 1:1 molecular complex with soluble recombinant human CD4 (rCD4) receptor, as demonstrated by gel-filtration HPLC; binding is high affinity (Kd ˜2 x 10^-9 M).

Original language	English (US)
Pages (from-to)	1233-1244
Number of pages	12
Journal	AIDS Research and Human Retroviruses
Volume	9
Issue number	12
DOIs	https://doi.org/10.1089/aid.1993.9.1233
State	Published - Dec 1993
Externally published	Yes

ASJC Scopus subject areas

Immunology
Virology
Infectious Diseases

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10.1089/aid.1993.9.1233

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Scandella, C. J., Kilpatrick, J., Lidster, W., Parker, C., Moore, J. P., Moore, G. K., Mann, K. A., Brown, P., Coates, S., Chapman, B., Masiarz, F. R., Steimer, K. S., & Haigwood, N. L. (1993). Nonaffinity Purification of Recombinant gp120 for Use in AIDS Vaccine Development. AIDS Research and Human Retroviruses, 9(12), 1233-1244. https://doi.org/10.1089/aid.1993.9.1233

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abstract = "The gene encoding the major envelope glycoprotein of the HIV-SF2 isolate was engineered for the secretion of recombinant gp120 (rgp120SF2) from permanent Chinese hamster ovary (CHO) cell lines. Cellular production methods were scaled up and a method for purification of the secreted glycoprotein was devised. Mild purification conditions were selected in order to preserve the native structure of the protein. rgp120SF2 exhibits a molecular weight of 120 kDa in reduced or nonreduced SDS gels; thus the polypeptide chain is intact. Deglycosylated rgp120SF2 has the predicted molecular weight of the polypeptide backbone, 54 kDa. Gelfiltration HPLC in a nondenaturing buffer at neutral pH yields a molecular weight estimate of approximately 120 kDa. Purified rgp120 closely resembles authentic viral gp120 by several physical, chemical, and immunochemical tests. rgp120SF2 reacts strongly with human HIV-positive sera, monoclonal antibodies reactive with HIV-SF2 and HIV-MN viral envelope, and a human virus-neutralizing monoclonal antibody that maps to a conserved discontinuous epitope on HIV-1 gp120. Purified rgp120SF2 forms a 1:1 molecular complex with soluble recombinant human CD4 (rCD4) receptor, as demonstrated by gel-filtration HPLC; binding is high affinity (Kd ˜2 x 10-9 M).",

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Nonaffinity Purification of Recombinant gp120 for Use in AIDS Vaccine Development

Abstract

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