TY - JOUR
T1 - Noninvasive diagnosis of intraamniotic infection
T2 - proteomic biomarkers in vaginal fluid
AU - Hitti, Jane
AU - Lapidus, Jodi A.
AU - Lu, Xinfang
AU - Reddy, Ashok P.
AU - Jacob, Thomas
AU - Dasari, Surendra
AU - Eschenbach, David A.
AU - Gravett, Michael G.
AU - Nagalla, Srinivasa R.
N1 - Funding Information:
Supported by National Institutes of Health Grant no. AI31871 . These analyses were supported by ProteoGenix, Inc.
PY - 2010/7
Y1 - 2010/7
N2 - Objective: We analyzed the vaginal fluid proteome to identify biomarkers of intraamniotic infection among women in preterm labor. Study Design: Proteome analysis was performed on vaginal fluid specimens from women with preterm labor, using multidimensional liquid chromatography, tandem mass spectrometry, and label-free quantification. Enzyme immunoassays were used to quantify candidate proteins. Classification accuracy for intraamniotic infection (positive amniotic fluid bacterial culture and/or interleukin-6 >2 ng/mL) was evaluated using receiver-operator characteristic curves obtained by logistic regression. Results: Of 170 subjects, 30 (18%) had intraamniotic infection. Vaginal fluid proteome analysis revealed 338 unique proteins. Label-free quantification identified 15 proteins differentially expressed in intraamniotic infection, including acute-phase reactants, immune modulators, high-abundance amniotic fluid proteins and extracellular matrix-signaling factors; these findings were confirmed by enzyme immunoassay. A multi-analyte algorithm showed accurate classification of intraamniotic infection. Conclusion: Vaginal fluid proteome analyses identified proteins capable of discriminating between patients with and without intraamniotic infection.
AB - Objective: We analyzed the vaginal fluid proteome to identify biomarkers of intraamniotic infection among women in preterm labor. Study Design: Proteome analysis was performed on vaginal fluid specimens from women with preterm labor, using multidimensional liquid chromatography, tandem mass spectrometry, and label-free quantification. Enzyme immunoassays were used to quantify candidate proteins. Classification accuracy for intraamniotic infection (positive amniotic fluid bacterial culture and/or interleukin-6 >2 ng/mL) was evaluated using receiver-operator characteristic curves obtained by logistic regression. Results: Of 170 subjects, 30 (18%) had intraamniotic infection. Vaginal fluid proteome analysis revealed 338 unique proteins. Label-free quantification identified 15 proteins differentially expressed in intraamniotic infection, including acute-phase reactants, immune modulators, high-abundance amniotic fluid proteins and extracellular matrix-signaling factors; these findings were confirmed by enzyme immunoassay. A multi-analyte algorithm showed accurate classification of intraamniotic infection. Conclusion: Vaginal fluid proteome analyses identified proteins capable of discriminating between patients with and without intraamniotic infection.
KW - intraamniotic infection
KW - preterm labor
KW - proteomics
KW - vaginal fluid
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U2 - 10.1016/j.ajog.2010.03.037
DO - 10.1016/j.ajog.2010.03.037
M3 - Article
C2 - 20478552
AN - SCOPUS:77953915771
SN - 0002-9378
VL - 203
SP - 32.e1-32.e8
JO - American Journal of Obstetrics and Gynecology
JF - American Journal of Obstetrics and Gynecology
IS - 1
ER -