TY - JOUR
T1 - Optical coherence tomography for in vivo imaging of endocardial to mesenchymal transition during avian heart development
AU - Courchaine, Katherine
AU - Rugonyi, Sandra
N1 - Funding Information:
National Heart, Lung, and Blood Institute (HL094570); Medical Research Foundation.
Publisher Copyright:
© 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
PY - 2019/11/1
Y1 - 2019/11/1
N2 - The endocardial to mesenchymal transition (EndMT) that occurs in endocardial cushions during heart development is critical for proper heart septation and formation of the heart’s valves. In EndMT, cells delaminate from the endocardium and migrate into the previously acellular endocardial cushions. Optical coherence tomography (OCT) imaging uses the optical properties of tissues for contrast, and during early development OCT can differentiate cellular versus acellular tissues. Here we show that OCT can be used to non-invasively track EndMT progression in vivo in the outflow tract cushions of chicken embryos. This enables in vivo studies to elucidate factors leading to cardiac malformations.
AB - The endocardial to mesenchymal transition (EndMT) that occurs in endocardial cushions during heart development is critical for proper heart septation and formation of the heart’s valves. In EndMT, cells delaminate from the endocardium and migrate into the previously acellular endocardial cushions. Optical coherence tomography (OCT) imaging uses the optical properties of tissues for contrast, and during early development OCT can differentiate cellular versus acellular tissues. Here we show that OCT can be used to non-invasively track EndMT progression in vivo in the outflow tract cushions of chicken embryos. This enables in vivo studies to elucidate factors leading to cardiac malformations.
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U2 - 10.1364/BOE.10.005989
DO - 10.1364/BOE.10.005989
M3 - Article
AN - SCOPUS:85078139237
SN - 2156-7085
VL - 10
SP - 5989
EP - 5995
JO - Biomedical Optics Express
JF - Biomedical Optics Express
IS - 11
ER -