Optical imaging of the peri-tumoral inflammatory response in breast cancer

Akhilesh K. Sista; Robert J. Knebel; Sidhartha Tavri; Magnus Johansson; David G. DeNardo; Sophie E. Boddington; Sirish A. Kishore; Celina Ansari; Verena Reinhart; Fergus V. Coakley; Lisa M. Coussens; Heike E. Daldrup-Link

doi:10.1186/1479-5876-7-94

Optical imaging of the peri-tumoral inflammatory response in breast cancer

Akhilesh K. Sista
, Robert J. Knebel
, Sidhartha Tavri
, Magnus Johansson
, David G. DeNardo
, Sophie E. Boddington
, Sirish A. Kishore
, Celina Ansari
, Verena Reinhart
, Fergus V. Coakley
, Lisa M. Coussens
, Heike E. Daldrup-Link

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Purpose: Peri-tumoral inflammation is a common tumor response that plays a central role in tumor invasion and metastasis, and inflammatory cell recruitment is essential to this process. The purpose of this study was to determine whether injected fluorescently-labeled monocytes accumulate within murine breast tumors and are visible with optical imaging. Materials and methods: Murine monocytes were labeled with the fluorescent dye DiD and subsequently injected intravenously into 6 transgenic MMTV-PymT tumor-bearing mice and 6 FVB/n control mice without tumors. Optical imaging (OI) was performed before and after cell injection. Ratios of post-injection to pre-injection fluorescent signal intensity of the tumors (MMTV-PymT mice) and mammary tissue (FVB/n controls) were calculated and statistically compared. Results: MMTV-PymT breast tumors had an average post/pre signal intensity ratio of 1.8+/- 0.2 (range 1.1-2.7). Control mammary tissue had an average post/pre signal intensity ratio of 1.1 +/- 0.1 (range, 0.4 to 1.4). The p-value for the difference between the ratios was less than 0.05. Confocal fluorescence microscopy confirmed the presence of DiD-labeled cells within the breast tumors. Conclusion: Murine monocytes accumulate at the site of breast cancer development in this transgenic model, providing evidence that peri-tumoral inflammatory cell recruitment can be evaluated non-invasively using optical imaging.

Original language	English (US)
Article number	94
Journal	Journal of translational medicine
Volume	7
DOIs	https://doi.org/10.1186/1479-5876-7-94
State	Published - Nov 11 2009
Externally published	Yes

Funding

Dr. Sista was supported by a T32 training grant from the National Institute of Biomedical Imaging and Bioengineering (NIBIB). Dr. Coussens was supported by grants from the National Institutes of Health (CA72006, CA94168, CA098075) and a Department of Defense Era of Hope Scholar Award (BC051640). The project described was also supported by Award Number R21CA129725 from the National Cancer Institute and a University of California San Francisco, Department of Radiology and Biomedical Imaging seed grant, #07-02.

Funders	Funder number
Department of Defense Era of Hope	BC051640
Author National Institutes of Health National Institutes of Health National Institutes of Health National Institutes of Health The Bev Hartig Huntington's Disease Foundation National Institutes of Health	CA098075, CA72006
National Institute of Health-National Cancer Institute	07-02, R01CA094168, R21CA129725
National Institute of Biomedical Imaging and Bioengineering

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

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10.1186/1479-5876-7-94

Cite this

@article{1411619dd189435199dac92b85c5db39,

title = "Optical imaging of the peri-tumoral inflammatory response in breast cancer",

abstract = "Purpose: Peri-tumoral inflammation is a common tumor response that plays a central role in tumor invasion and metastasis, and inflammatory cell recruitment is essential to this process. The purpose of this study was to determine whether injected fluorescently-labeled monocytes accumulate within murine breast tumors and are visible with optical imaging. Materials and methods: Murine monocytes were labeled with the fluorescent dye DiD and subsequently injected intravenously into 6 transgenic MMTV-PymT tumor-bearing mice and 6 FVB/n control mice without tumors. Optical imaging (OI) was performed before and after cell injection. Ratios of post-injection to pre-injection fluorescent signal intensity of the tumors (MMTV-PymT mice) and mammary tissue (FVB/n controls) were calculated and statistically compared. Results: MMTV-PymT breast tumors had an average post/pre signal intensity ratio of 1.8+/- 0.2 (range 1.1-2.7). Control mammary tissue had an average post/pre signal intensity ratio of 1.1 +/- 0.1 (range, 0.4 to 1.4). The p-value for the difference between the ratios was less than 0.05. Confocal fluorescence microscopy confirmed the presence of DiD-labeled cells within the breast tumors. Conclusion: Murine monocytes accumulate at the site of breast cancer development in this transgenic model, providing evidence that peri-tumoral inflammatory cell recruitment can be evaluated non-invasively using optical imaging.",

author = "Sista, \{Akhilesh K.\} and Knebel, \{Robert J.\} and Sidhartha Tavri and Magnus Johansson and DeNardo, \{David G.\} and Boddington, \{Sophie E.\} and Kishore, \{Sirish A.\} and Celina Ansari and Verena Reinhart and Coakley, \{Fergus V.\} and Coussens, \{Lisa M.\} and Daldrup-Link, \{Heike E.\}",

note = "Funding Information: Dr. Sista was supported by a T32 training grant from the National Institute of Biomedical Imaging and Bioengineering (NIBIB). Dr. Coussens was supported by grants from the National Institutes of Health (CA72006, CA94168, CA098075) and a Department of Defense Era of Hope Scholar Award (BC051640). The project described was also supported by Award Number R21CA129725 from the National Cancer Institute and a University of California San Francisco, Department of Radiology and Biomedical Imaging seed grant, \#07-02.",

year = "2009",

month = nov,

day = "11",

doi = "10.1186/1479-5876-7-94",

language = "English (US)",

volume = "7",

journal = "Journal of translational medicine",

issn = "1479-5876",

publisher = "BioMed Central",

}

TY - JOUR

T1 - Optical imaging of the peri-tumoral inflammatory response in breast cancer

AU - Sista, Akhilesh K.

AU - Knebel, Robert J.

AU - Tavri, Sidhartha

AU - Johansson, Magnus

AU - DeNardo, David G.

AU - Boddington, Sophie E.

AU - Kishore, Sirish A.

AU - Ansari, Celina

AU - Reinhart, Verena

AU - Coakley, Fergus V.

AU - Coussens, Lisa M.

AU - Daldrup-Link, Heike E.

N1 - Funding Information: Dr. Sista was supported by a T32 training grant from the National Institute of Biomedical Imaging and Bioengineering (NIBIB). Dr. Coussens was supported by grants from the National Institutes of Health (CA72006, CA94168, CA098075) and a Department of Defense Era of Hope Scholar Award (BC051640). The project described was also supported by Award Number R21CA129725 from the National Cancer Institute and a University of California San Francisco, Department of Radiology and Biomedical Imaging seed grant, #07-02.

PY - 2009/11/11

Y1 - 2009/11/11

N2 - Purpose: Peri-tumoral inflammation is a common tumor response that plays a central role in tumor invasion and metastasis, and inflammatory cell recruitment is essential to this process. The purpose of this study was to determine whether injected fluorescently-labeled monocytes accumulate within murine breast tumors and are visible with optical imaging. Materials and methods: Murine monocytes were labeled with the fluorescent dye DiD and subsequently injected intravenously into 6 transgenic MMTV-PymT tumor-bearing mice and 6 FVB/n control mice without tumors. Optical imaging (OI) was performed before and after cell injection. Ratios of post-injection to pre-injection fluorescent signal intensity of the tumors (MMTV-PymT mice) and mammary tissue (FVB/n controls) were calculated and statistically compared. Results: MMTV-PymT breast tumors had an average post/pre signal intensity ratio of 1.8+/- 0.2 (range 1.1-2.7). Control mammary tissue had an average post/pre signal intensity ratio of 1.1 +/- 0.1 (range, 0.4 to 1.4). The p-value for the difference between the ratios was less than 0.05. Confocal fluorescence microscopy confirmed the presence of DiD-labeled cells within the breast tumors. Conclusion: Murine monocytes accumulate at the site of breast cancer development in this transgenic model, providing evidence that peri-tumoral inflammatory cell recruitment can be evaluated non-invasively using optical imaging.

AB - Purpose: Peri-tumoral inflammation is a common tumor response that plays a central role in tumor invasion and metastasis, and inflammatory cell recruitment is essential to this process. The purpose of this study was to determine whether injected fluorescently-labeled monocytes accumulate within murine breast tumors and are visible with optical imaging. Materials and methods: Murine monocytes were labeled with the fluorescent dye DiD and subsequently injected intravenously into 6 transgenic MMTV-PymT tumor-bearing mice and 6 FVB/n control mice without tumors. Optical imaging (OI) was performed before and after cell injection. Ratios of post-injection to pre-injection fluorescent signal intensity of the tumors (MMTV-PymT mice) and mammary tissue (FVB/n controls) were calculated and statistically compared. Results: MMTV-PymT breast tumors had an average post/pre signal intensity ratio of 1.8+/- 0.2 (range 1.1-2.7). Control mammary tissue had an average post/pre signal intensity ratio of 1.1 +/- 0.1 (range, 0.4 to 1.4). The p-value for the difference between the ratios was less than 0.05. Confocal fluorescence microscopy confirmed the presence of DiD-labeled cells within the breast tumors. Conclusion: Murine monocytes accumulate at the site of breast cancer development in this transgenic model, providing evidence that peri-tumoral inflammatory cell recruitment can be evaluated non-invasively using optical imaging.

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UR - https://www.scopus.com/pages/publications/71049158817#tab=citedBy

U2 - 10.1186/1479-5876-7-94

DO - 10.1186/1479-5876-7-94

M3 - Article

C2 - 19906309

AN - SCOPUS:71049158817

SN - 1479-5876

VL - 7

JO - Journal of translational medicine

JF - Journal of translational medicine

M1 - 94

ER -

Optical imaging of the peri-tumoral inflammatory response in breast cancer

Abstract

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ASJC Scopus subject areas

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