Optimization and use of near infrared imaging to guide lymph node collection in rhesus macaques (Macaca mulatta)

Jeremy V. Smedley; Rachele M. Bochart; Miranda Fischer; Heidi Funderburgh; Vanessa Kelly; Hugh Crank; Kim Armantrout; Oriene Shiel; Mitchell Robertson-LeVay; Nikki Sternberger; Brian Schmaling; Sheila Roberts; Vicki Sekiguchi; Michael Reusz; Tiah Schwartz; Kimberly A. Meyer; Gabriela Webb; Roxanne M. Gilbride; Nicholas Dambrauskas; Daniela Andrade; Matthew Wood; Caralyn Labriola; Michael Axthelm; Nina Derby; Ben Varco-Merth; Yoshinori Fukazawa; Scott Hansen; Jonah B. Sacha; Donald L Sodora; D. Noah Sather

doi:10.1111/jmp.12605

Optimization and use of near infrared imaging to guide lymph node collection in rhesus macaques (Macaca mulatta)

Jeremy V. Smedley, Rachele M. Bochart, Miranda Fischer, Heidi Funderburgh, Vanessa Kelly, Hugh Crank, Kim Armantrout, Oriene Shiel, Mitchell Robertson-LeVay, Nikki Sternberger, Brian Schmaling, Sheila Roberts, Vicki Sekiguchi, Michael Reusz, Tiah Schwartz, Kimberly A. Meyer, Gabriela Webb, Roxanne M. Gilbride, Nicholas Dambrauskas, Daniela AndradeMatthew Wood, Caralyn Labriola, Michael Axthelm, Nina Derby, Ben Varco-Merth, Yoshinori Fukazawa, Scott Hansen, Jonah B. Sacha, Donald L Sodora, D. Noah Sather

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Background: Identification of lymph nodes (LNs) draining a specific site or in obese macaques can be challenging. Methods: Indocyanine Green (ICG) was administered intradermal (ID), intramuscular, in the oral mucosa, or subserosal in the colon followed by Near Infrared (NIR) imaging. Results: After optimization to maximize LN identification, intradermal ICG was successful in identifying 50–100% of the axillary/inguinal LN at a site. Using NIR, collection of peripheral and mesenteric LNs in obese macaques was 100% successful after traditional methods failed. Additionally, guided collection of LNs draining the site of intraepithelial or intramuscular immunization demonstrated significantly increased numbers of T follicular helper (Tfh) cells in germinal centers of draining compared to nondraining LNs. Conclusion: These imaging techniques optimize our ability to evaluate immune changes within LNs over time, even in obese macaques. This approach allows for targeted serial biopsies that permit confidence that draining LNs are being harvested throughout the study.

Original language	English (US)
Pages (from-to)	270-277
Number of pages	8
Journal	Journal of medical primatology
Volume	51
Issue number	5
DOIs	https://doi.org/10.1111/jmp.12605
State	Published - Oct 2022

Keywords

ICG
MLN
draining
fluorescence
imaging
indocyanine green
lymph node
macaque
mesenteric
targeted sampling

ASJC Scopus subject areas

Animal Science and Zoology
General Veterinary

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10.1111/jmp.12605

Cite this

Smedley, J. V., Bochart, R. M., Fischer, M., Funderburgh, H., Kelly, V., Crank, H., Armantrout, K., Shiel, O., Robertson-LeVay, M., Sternberger, N., Schmaling, B., Roberts, S., Sekiguchi, V., Reusz, M., Schwartz, T., Meyer, K. A., Webb, G., Gilbride, R. M., Dambrauskas, N., ... Sather, D. N. (2022). Optimization and use of near infrared imaging to guide lymph node collection in rhesus macaques (Macaca mulatta). Journal of medical primatology, 51(5), 270-277. https://doi.org/10.1111/jmp.12605

Smedley, JV, Bochart, RM, Fischer, M, Funderburgh, H, Kelly, V, Crank, H, Armantrout, K, Shiel, O, Robertson-LeVay, M, Sternberger, N, Schmaling, B, Roberts, S, Sekiguchi, V, Reusz, M, Schwartz, T, Meyer, KA, Webb, G, Gilbride, RM, Dambrauskas, N, Andrade, D, Wood, M, Labriola, C , Axthelm, M, Derby, N, Varco-Merth, B, Fukazawa, Y, Hansen, S , Sacha, JB, Sodora, DL & Sather, DN 2022, 'Optimization and use of near infrared imaging to guide lymph node collection in rhesus macaques (Macaca mulatta)', Journal of medical primatology, vol. 51, no. 5, pp. 270-277. https://doi.org/10.1111/jmp.12605

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title = "Optimization and use of near infrared imaging to guide lymph node collection in rhesus macaques (Macaca mulatta)",

abstract = "Background: Identification of lymph nodes (LNs) draining a specific site or in obese macaques can be challenging. Methods: Indocyanine Green (ICG) was administered intradermal (ID), intramuscular, in the oral mucosa, or subserosal in the colon followed by Near Infrared (NIR) imaging. Results: After optimization to maximize LN identification, intradermal ICG was successful in identifying 50–100% of the axillary/inguinal LN at a site. Using NIR, collection of peripheral and mesenteric LNs in obese macaques was 100% successful after traditional methods failed. Additionally, guided collection of LNs draining the site of intraepithelial or intramuscular immunization demonstrated significantly increased numbers of T follicular helper (Tfh) cells in germinal centers of draining compared to nondraining LNs. Conclusion: These imaging techniques optimize our ability to evaluate immune changes within LNs over time, even in obese macaques. This approach allows for targeted serial biopsies that permit confidence that draining LNs are being harvested throughout the study.",

keywords = "ICG, MLN, draining, fluorescence, imaging, indocyanine green, lymph node, macaque, mesenteric, targeted sampling",

author = "Smedley, {Jeremy V.} and Bochart, {Rachele M.} and Miranda Fischer and Heidi Funderburgh and Vanessa Kelly and Hugh Crank and Kim Armantrout and Oriene Shiel and Mitchell Robertson-LeVay and Nikki Sternberger and Brian Schmaling and Sheila Roberts and Vicki Sekiguchi and Michael Reusz and Tiah Schwartz and Meyer, {Kimberly A.} and Gabriela Webb and Gilbride, {Roxanne M.} and Nicholas Dambrauskas and Daniela Andrade and Matthew Wood and Caralyn Labriola and Michael Axthelm and Nina Derby and Ben Varco-Merth and Yoshinori Fukazawa and Scott Hansen and Sacha, {Jonah B.} and Donald L Sodora and Sather, {D. Noah}",

note = "Publisher Copyright: {\textcopyright} 2022 The Authors. Journal of Medical Primatology published by John Wiley & Sons Ltd.",

year = "2022",

month = oct,

doi = "10.1111/jmp.12605",

language = "English (US)",

volume = "51",

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T1 - Optimization and use of near infrared imaging to guide lymph node collection in rhesus macaques (Macaca mulatta)

AU - Smedley, Jeremy V.

AU - Bochart, Rachele M.

AU - Fischer, Miranda

AU - Funderburgh, Heidi

AU - Kelly, Vanessa

AU - Crank, Hugh

AU - Armantrout, Kim

AU - Shiel, Oriene

AU - Robertson-LeVay, Mitchell

AU - Sternberger, Nikki

AU - Schmaling, Brian

AU - Roberts, Sheila

AU - Sekiguchi, Vicki

AU - Reusz, Michael

AU - Schwartz, Tiah

AU - Meyer, Kimberly A.

AU - Webb, Gabriela

AU - Gilbride, Roxanne M.

AU - Dambrauskas, Nicholas

AU - Andrade, Daniela

AU - Wood, Matthew

AU - Labriola, Caralyn

AU - Axthelm, Michael

AU - Derby, Nina

AU - Varco-Merth, Ben

AU - Fukazawa, Yoshinori

AU - Hansen, Scott

AU - Sacha, Jonah B.

AU - Sodora, Donald L

AU - Sather, D. Noah

PY - 2022/10

Y1 - 2022/10

N2 - Background: Identification of lymph nodes (LNs) draining a specific site or in obese macaques can be challenging. Methods: Indocyanine Green (ICG) was administered intradermal (ID), intramuscular, in the oral mucosa, or subserosal in the colon followed by Near Infrared (NIR) imaging. Results: After optimization to maximize LN identification, intradermal ICG was successful in identifying 50–100% of the axillary/inguinal LN at a site. Using NIR, collection of peripheral and mesenteric LNs in obese macaques was 100% successful after traditional methods failed. Additionally, guided collection of LNs draining the site of intraepithelial or intramuscular immunization demonstrated significantly increased numbers of T follicular helper (Tfh) cells in germinal centers of draining compared to nondraining LNs. Conclusion: These imaging techniques optimize our ability to evaluate immune changes within LNs over time, even in obese macaques. This approach allows for targeted serial biopsies that permit confidence that draining LNs are being harvested throughout the study.

AB - Background: Identification of lymph nodes (LNs) draining a specific site or in obese macaques can be challenging. Methods: Indocyanine Green (ICG) was administered intradermal (ID), intramuscular, in the oral mucosa, or subserosal in the colon followed by Near Infrared (NIR) imaging. Results: After optimization to maximize LN identification, intradermal ICG was successful in identifying 50–100% of the axillary/inguinal LN at a site. Using NIR, collection of peripheral and mesenteric LNs in obese macaques was 100% successful after traditional methods failed. Additionally, guided collection of LNs draining the site of intraepithelial or intramuscular immunization demonstrated significantly increased numbers of T follicular helper (Tfh) cells in germinal centers of draining compared to nondraining LNs. Conclusion: These imaging techniques optimize our ability to evaluate immune changes within LNs over time, even in obese macaques. This approach allows for targeted serial biopsies that permit confidence that draining LNs are being harvested throughout the study.

KW - ICG

KW - MLN

KW - draining

KW - fluorescence

KW - imaging

KW - indocyanine green

KW - lymph node

KW - macaque

KW - mesenteric

KW - targeted sampling

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C2 - 35841132

AN - SCOPUS:85134048848

SN - 0047-2565

VL - 51

SP - 270

EP - 277

JO - Journal of medical primatology

JF - Journal of medical primatology

IS - 5

ER -

Optimization and use of near infrared imaging to guide lymph node collection in rhesus macaques (Macaca mulatta)

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