TY - JOUR
T1 - Oral microbiota species in acute apical endodontic abscesses
AU - George, Noelle
AU - Flamiatos, Erin
AU - Kawasaki, Kellie
AU - Kim, Namgu
AU - Carriere, Charles
AU - Phan, Brian
AU - Joseph, Raphael
AU - Strauss, Shay
AU - Kohli, Richie
AU - Choi, Dongseok
AU - Craig Baumgartner, J.
AU - Sedgley, Christine
AU - Maier, Tom
AU - Machida, Curtis A.
N1 - Funding Information:
NG and EF are noted as equal contributors to this work. This work was originally supported in part by a grant from the American Association of Endodontists Foundation (to CM). NG is a recipient of the 2015ADA/Dentsply Student ClinicianResearchAward. EFis a recipient of the Dean's Student Research Fellowship Award from the OHSU School of Dentistry. NG, EF, KK, NK, CC, BP, and RJ are enrolled in theDMDprogram at theOHSUSchool of Dentistry. SS is a clinical study coordinator for our program at the OHSU School of Dentistry. RK, CS, TM, and CM receive support from the OHSU School of Dentistry. DC and CM are also supported in part by NIH DE024317. We thank Truman Nielsen for support in conducting earlier portions of the experimentation. We also thank Dr. Bruce J. Paster for providing detailed commentary concerning the HOMIM probe generation, hybridization, and washing procedures. There is no conflict of interest in the present study for any of the authors.
Publisher Copyright:
© 2016 Noelle George et al.
PY - 2016
Y1 - 2016
N2 - Background and objectives: Acute apical abscesses are serious endodontic diseases resulting from pulpal infection with opportunistic oral microorganisms. The objective of this study was to identify and compare the oral microbiota in patients (N=18) exhibiting acute apical abscesses, originating from the demographic region in Portland, Oregon. The study hypothesis is that abscesses obtained from this demographic region may contain unique microorganisms not identified in specimens from other regions. Design: Endodontic abscesses were sampled from patients at the Oregon Health & Science University (OHSU) School of Dentistry. DNA from abscess specimens was subjected to polymerase chain reaction amplification using 16S rRNA gene-specific primers and Cy3-dCTP labeling. Labeled DNAwas then applied to microbial microarrays (280 species) generated by the Human Oral Microbial Identification Microarray Laboratory (Forsyth Institute, Cambridge, MA). Results: The most prevalent microorganisms, found across multiple abscess specimens, include Fusobacterium nucleatum, Parvimonas micra, Megasphaera species clone CS025, Prevotella multisaccharivorax, Atopobium rimae, and Porphyromonas endodontalis. The most abundant microorganisms, found in highest numbers within individual abscesses, include F. nucleatum, P. micra, Streptococcus Cluster III, Solobacterium moorei, Streptococcus constellatus, and Porphyromonas endodontalis. Strong bacterial associations were identified between Prevotella multisaccharivorax, Acidaminococcaceae species clone DM071, Megasphaera species clone CS025, Actinomyces species clone EP053, and Streptococcus cristatus (all with Spearman coefficients > 0.9). Conclusions: Cultivable and uncultivable bacterial species have been identified in endodontic abscesses obtained from the Portland, Oregon demographic region, and taxa identifications correlated well with other published studies, with the exception of Treponema and Streptococcus cristae, which were not commonly identified in endodontic abscesses between the demographic region in Portland, Oregon and other regions.
AB - Background and objectives: Acute apical abscesses are serious endodontic diseases resulting from pulpal infection with opportunistic oral microorganisms. The objective of this study was to identify and compare the oral microbiota in patients (N=18) exhibiting acute apical abscesses, originating from the demographic region in Portland, Oregon. The study hypothesis is that abscesses obtained from this demographic region may contain unique microorganisms not identified in specimens from other regions. Design: Endodontic abscesses were sampled from patients at the Oregon Health & Science University (OHSU) School of Dentistry. DNA from abscess specimens was subjected to polymerase chain reaction amplification using 16S rRNA gene-specific primers and Cy3-dCTP labeling. Labeled DNAwas then applied to microbial microarrays (280 species) generated by the Human Oral Microbial Identification Microarray Laboratory (Forsyth Institute, Cambridge, MA). Results: The most prevalent microorganisms, found across multiple abscess specimens, include Fusobacterium nucleatum, Parvimonas micra, Megasphaera species clone CS025, Prevotella multisaccharivorax, Atopobium rimae, and Porphyromonas endodontalis. The most abundant microorganisms, found in highest numbers within individual abscesses, include F. nucleatum, P. micra, Streptococcus Cluster III, Solobacterium moorei, Streptococcus constellatus, and Porphyromonas endodontalis. Strong bacterial associations were identified between Prevotella multisaccharivorax, Acidaminococcaceae species clone DM071, Megasphaera species clone CS025, Actinomyces species clone EP053, and Streptococcus cristatus (all with Spearman coefficients > 0.9). Conclusions: Cultivable and uncultivable bacterial species have been identified in endodontic abscesses obtained from the Portland, Oregon demographic region, and taxa identifications correlated well with other published studies, with the exception of Treponema and Streptococcus cristae, which were not commonly identified in endodontic abscesses between the demographic region in Portland, Oregon and other regions.
KW - Anaerobic oral microorganisms
KW - Endodontic abscesses
KW - Fusobacterium nucleatum
KW - Human oral microbial identification microarrays
KW - Oral microbiota
KW - Streptococcus cristatus
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U2 - 10.3402/jom.v8.30989
DO - 10.3402/jom.v8.30989
M3 - Article
AN - SCOPUS:84997054335
SN - 2000-2297
VL - 8
JO - Journal of Oral Microbiology
JF - Journal of Oral Microbiology
IS - 1
M1 - 30989
ER -