PC12 cell aggregation and dopamine production on EHS-derived extracellular matrix

Cynthia L. Bethea; Thomas K. Borg

doi:10.1016/0303-7207(88)90145-1

PC12 cell aggregation and dopamine production on EHS-derived extracellular matrix

Cynthia L. Bethea, Thomas K. Borg

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

PC12 cells on plastic grow as a single-layered lawn of cells which synthesize, store and secrete dopamine. In contrast, PC12 cells cultured on Englebreth-Holm-Swarm (EHS) tumor-derived extracellular matrix grow into multicellular aggregates. Matrix dissolution and cell migration appear to follow aggregate formation. PC12 cell plating efficiency is decreased on EHS-matrix but the doubling time of cells on EHS-matrix is comparable to plastic. Dopamine secretion and cellular content determined with a radioenzymatic assay as well as dopamine synthesis determined with cation-exchange chromatography are similar on a per cell basis in cultures of PC12 cells on plastic and EHS-matrix.

Original language	English (US)
Pages (from-to)	113-128
Number of pages	16
Journal	Molecular and Cellular Endocrinology
Volume	58
Issue number	2-3
DOIs	https://doi.org/10.1016/0303-7207(88)90145-1
State	Published - Aug 1988
Externally published	Yes

Keywords

Dopamine
PC12 cells, Extracellular matrix

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Endocrinology

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10.1016/0303-7207(88)90145-1

Cite this

@article{8519cef09c4e4582b48dc1e94ea4228d,

title = "PC12 cell aggregation and dopamine production on EHS-derived extracellular matrix",

abstract = "PC12 cells on plastic grow as a single-layered lawn of cells which synthesize, store and secrete dopamine. In contrast, PC12 cells cultured on Englebreth-Holm-Swarm (EHS) tumor-derived extracellular matrix grow into multicellular aggregates. Matrix dissolution and cell migration appear to follow aggregate formation. PC12 cell plating efficiency is decreased on EHS-matrix but the doubling time of cells on EHS-matrix is comparable to plastic. Dopamine secretion and cellular content determined with a radioenzymatic assay as well as dopamine synthesis determined with cation-exchange chromatography are similar on a per cell basis in cultures of PC12 cells on plastic and EHS-matrix.",

keywords = "Dopamine, PC12 cells, Extracellular matrix",

author = "Bethea, {Cynthia L.} and Borg, {Thomas K.}",

note = "Funding Information: Address for correspondence: Dr. Cynthia L. Bethea. Oregon Regional Primate Research Center, 505 N.W. 185th Ave.. Beaverton. OR 97006, U.S.A. Publication No. 1568 of the Oregon Regional Primate Research Center. Supported by NIH grants DK 35044 to C.L.B., HL 24935 to T.K.B., P30 program project grant HD 18185. and RR 00163 for operation of the ORPRC.",

year = "1988",

month = aug,

doi = "10.1016/0303-7207(88)90145-1",

language = "English (US)",

volume = "58",

pages = "113--128",

journal = "Molecular and Cellular Endocrinology",

issn = "0303-7207",

publisher = "Elsevier Ireland Ltd",

number = "2-3",

}

TY - JOUR

T1 - PC12 cell aggregation and dopamine production on EHS-derived extracellular matrix

AU - Bethea, Cynthia L.

AU - Borg, Thomas K.

N1 - Funding Information: Address for correspondence: Dr. Cynthia L. Bethea. Oregon Regional Primate Research Center, 505 N.W. 185th Ave.. Beaverton. OR 97006, U.S.A. Publication No. 1568 of the Oregon Regional Primate Research Center. Supported by NIH grants DK 35044 to C.L.B., HL 24935 to T.K.B., P30 program project grant HD 18185. and RR 00163 for operation of the ORPRC.

PY - 1988/8

Y1 - 1988/8

N2 - PC12 cells on plastic grow as a single-layered lawn of cells which synthesize, store and secrete dopamine. In contrast, PC12 cells cultured on Englebreth-Holm-Swarm (EHS) tumor-derived extracellular matrix grow into multicellular aggregates. Matrix dissolution and cell migration appear to follow aggregate formation. PC12 cell plating efficiency is decreased on EHS-matrix but the doubling time of cells on EHS-matrix is comparable to plastic. Dopamine secretion and cellular content determined with a radioenzymatic assay as well as dopamine synthesis determined with cation-exchange chromatography are similar on a per cell basis in cultures of PC12 cells on plastic and EHS-matrix.

AB - PC12 cells on plastic grow as a single-layered lawn of cells which synthesize, store and secrete dopamine. In contrast, PC12 cells cultured on Englebreth-Holm-Swarm (EHS) tumor-derived extracellular matrix grow into multicellular aggregates. Matrix dissolution and cell migration appear to follow aggregate formation. PC12 cell plating efficiency is decreased on EHS-matrix but the doubling time of cells on EHS-matrix is comparable to plastic. Dopamine secretion and cellular content determined with a radioenzymatic assay as well as dopamine synthesis determined with cation-exchange chromatography are similar on a per cell basis in cultures of PC12 cells on plastic and EHS-matrix.

KW - Dopamine

KW - PC12 cells, Extracellular matrix

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UR - http://www.scopus.com/inward/citedby.url?scp=0023722284&partnerID=8YFLogxK

U2 - 10.1016/0303-7207(88)90145-1

DO - 10.1016/0303-7207(88)90145-1

M3 - Article

C2 - 3061856

AN - SCOPUS:0023722284

SN - 0303-7207

VL - 58

SP - 113

EP - 128

JO - Molecular and Cellular Endocrinology

JF - Molecular and Cellular Endocrinology

IS - 2-3

ER -

PC12 cell aggregation and dopamine production on EHS-derived extracellular matrix

Abstract

Keywords

ASJC Scopus subject areas

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