PC12 cell aggregation and dopamine production on EHS-derived extracellular matrix

Cynthia L. Bethea, Thomas K. Borg

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


PC12 cells on plastic grow as a single-layered lawn of cells which synthesize, store and secrete dopamine. In contrast, PC12 cells cultured on Englebreth-Holm-Swarm (EHS) tumor-derived extracellular matrix grow into multicellular aggregates. Matrix dissolution and cell migration appear to follow aggregate formation. PC12 cell plating efficiency is decreased on EHS-matrix but the doubling time of cells on EHS-matrix is comparable to plastic. Dopamine secretion and cellular content determined with a radioenzymatic assay as well as dopamine synthesis determined with cation-exchange chromatography are similar on a per cell basis in cultures of PC12 cells on plastic and EHS-matrix.

Original languageEnglish (US)
Pages (from-to)113-128
Number of pages16
JournalMolecular and Cellular Endocrinology
Issue number2-3
StatePublished - Aug 1988
Externally publishedYes


  • Dopamine
  • PC12 cells, Extracellular matrix

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology


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