Abstract
PC12 cells on plastic grow as a single-layered lawn of cells which synthesize, store and secrete dopamine. In contrast, PC12 cells cultured on Englebreth-Holm-Swarm (EHS) tumor-derived extracellular matrix grow into multicellular aggregates. Matrix dissolution and cell migration appear to follow aggregate formation. PC12 cell plating efficiency is decreased on EHS-matrix but the doubling time of cells on EHS-matrix is comparable to plastic. Dopamine secretion and cellular content determined with a radioenzymatic assay as well as dopamine synthesis determined with cation-exchange chromatography are similar on a per cell basis in cultures of PC12 cells on plastic and EHS-matrix.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 113-128 |
| Number of pages | 16 |
| Journal | Molecular and Cellular Endocrinology |
| Volume | 58 |
| Issue number | 2-3 |
| DOIs | |
| State | Published - Aug 1988 |
Funding
Address for correspondence: Dr. Cynthia L. Bethea. Oregon Regional Primate Research Center, 505 N.W. 185th Ave.. Beaverton. OR 97006, U.S.A. Publication No. 1568 of the Oregon Regional Primate Research Center. Supported by NIH grants DK 35044 to C.L.B., HL 24935 to T.K.B., P30 program project grant HD 18185. and RR 00163 for operation of the ORPRC.
| Funders | Funder number |
|---|---|
| Author National Institutes of Health National Institutes of Health National Institutes of Health National Institutes of Health The Bev Hartig Huntington's Disease Foundation National Institutes of Health | HD 18185, HL 24935 |
| National Institute of Diabetes and Digestive and Kidney Diseases | R01DK035044 |
Keywords
- Dopamine
- PC12 cells, Extracellular matrix
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Endocrinology
Fingerprint
Dive into the research topics of 'PC12 cell aggregation and dopamine production on EHS-derived extracellular matrix'. Together they form a unique fingerprint.Cite this
- APA
- Standard
- Harvard
- Vancouver
- Author
- BIBTEX
- RIS