@article{696ba9dfa2114bf18afbbb18a9f1aa34,
title = "Peripheral nerve development in zebrafish requires muscle patterning by tcf15/paraxis",
abstract = "The vertebrate peripheral nervous system (PNS) is an intricate network that conveys sensory and motor information throughout the body. During development, extracellular cues direct the migration of axons and glia through peripheral tissues. Currently, the suite of molecules that govern PNS axon-glial patterning is incompletely understood. To elucidate factors that are critical for peripheral nerve development, we characterized the novel zebrafish mutant, stl159, that exhibits abnormalities in PNS patterning. In these mutants, motor and sensory nerves that develop adjacent to axial muscle fail to extend normally, and neuromasts in the posterior lateral line system, as well as neural crest-derived melanocytes, are incorrectly positioned. The stl159 genetic lesion lies in the basic helix-loop-helix (bHLH) transcription factor tcf15, which has been previously implicated in proper development of axial muscles. We find that targeted loss of tcf15 via CRISPR-Cas9 genome editing results in the PNS patterning abnormalities observed in stl159 mutants. Because tcf15 is expressed in developing muscle prior to nerve extension, rather than in neurons or glia, we predict that tcf15 non-cell-autonomously promotes peripheral nerve patterning in zebrafish through regulation of extracellular patterning cues. Our work underscores the importance of muscle-derived factors in PNS development.",
keywords = "Lateral line nerve, Myelin, Paraxial mesoderm, Schwann cells, Zebrafish",
author = "Limbach, {Lauren E.} and Penick, {Rocky L.} and Casseday, {Rudy S.} and Hyland, {Maddelyn A.} and Pontillo, {Erika A.} and Ayele, {Afomia N.} and Pitts, {Kristen M.} and Ackerman, {Sarah D.} and Harty, {Breanne L.} and Herbert, {Amy L.} and Monk, {Kelly R.} and Petersen, {Sarah C.}",
note = "Funding Information: We thank our colleagues at Kenyon College and members of the Petersen laboratory for critical feedback, thoughtful discussion, and generosity with equipment and reagents. We thank Rebecca Cunningham, Lila Solnica-Krezel, and members of the Monk and Solnica-Krezel laboratories at Washington University in St. Louis for reagents and the initial identification of the stl159 allele, as well as Sarah Kucenas and Cody Smith for additional zebrafish transgenic strains. We thank Becky Gallagher and the animal care staff at Kenyon College for excellent zebrafish care. This work was supported by NIH F32 NS087786 and NSF BIO-IOS # 1941664 to S.C.P., NIH R01 HD080601 to K.R.M., and Kenyon College Summer Science Scholars fellowships to E.A.P., A.A., and K.P. Funding Information: We thank our colleagues at Kenyon College and members of the Petersen laboratory for critical feedback, thoughtful discussion, and generosity with equipment and reagents. We thank Rebecca Cunningham, Lila Solnica-Krezel, and members of the Monk and Solnica-Krezel laboratories at Washington University in St. Louis for reagents and the initial identification of the stl159 allele, as well as Sarah Kucenas and Cody Smith for additional zebrafish transgenic strains. We thank Becky Gallagher and the animal care staff at Kenyon College for excellent zebrafish care. This work was supported by NIH F32 NS087786 and NSF BIO-IOS #1941664 to S.C.P. NIH R01 HD080601 to K.R.M. and Kenyon College Summer Science Scholars fellowships to E.A.P. A.A. and K.P. Publisher Copyright: {\textcopyright} 2022 Elsevier Inc.",
year = "2022",
month = oct,
doi = "10.1016/j.ydbio.2022.07.001",
language = "English (US)",
volume = "490",
pages = "37--49",
journal = "Developmental Biology",
issn = "0012-1606",
publisher = "Academic Press Inc.",
}