TY - JOUR
T1 - Phosphate analogs block adaptation in hair cells by inhibiting adaptation-motor force production
AU - Yamoah, Ebenezer N.
AU - Gillespie, Peter G.
N1 - Funding Information:
We appreciate the constructive comments on the manuscript that were provided by Fernan Jaramillo and by members of the Gillespie laboratory. This work was supported by the National Institutes of Health (R01 DC02368 and P60 DC00979). P. G. G. is a Pew Scholar in the Biomedical Sciences. Correspondence should be addressed to P. G. G.
PY - 1996/9
Y1 - 1996/9
N2 - To ensure optimal sensitivity for mechanoelectrical transduction, hair cells adapt to prolonged stimuli using active motors. Adaptation motors are thought to employ myosin molecules as their force-producing components. We find that beryllium fluoride, vanadate, and sulfate, phosphate analogs that inhibit the ATPase activity of myosin, inhibit adaptation by abolishing motor force production. Phosphate analogs interact with a 120-kDa bundle protein, most likely myosin Iβ, in a manner that coincides with their effects on adaptation. Features of transduction following inhibition of motor force production suggest that the gating and extent springs of the hair cell orient in parallel at rest and that the negative limit of adaptation arises when force in the stretched extent spring matches the force output of the adaptation motor.
AB - To ensure optimal sensitivity for mechanoelectrical transduction, hair cells adapt to prolonged stimuli using active motors. Adaptation motors are thought to employ myosin molecules as their force-producing components. We find that beryllium fluoride, vanadate, and sulfate, phosphate analogs that inhibit the ATPase activity of myosin, inhibit adaptation by abolishing motor force production. Phosphate analogs interact with a 120-kDa bundle protein, most likely myosin Iβ, in a manner that coincides with their effects on adaptation. Features of transduction following inhibition of motor force production suggest that the gating and extent springs of the hair cell orient in parallel at rest and that the negative limit of adaptation arises when force in the stretched extent spring matches the force output of the adaptation motor.
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U2 - 10.1016/S0896-6273(00)80184-1
DO - 10.1016/S0896-6273(00)80184-1
M3 - Article
C2 - 8816715
AN - SCOPUS:0030245782
SN - 0896-6273
VL - 17
SP - 523
EP - 533
JO - Neuron
JF - Neuron
IS - 3
ER -