@article{67cdaf6578b5480b9648d4b55dcd9853,
title = "Phosphatidylinositol 3-kinase: Structure and expression of the 110 kd catalytic subunit",
abstract = "Purified bovine brain phosphatidylinositol 3-kinase (PI3-kinase) is composed of 85 kd and 110 kd subunits. The 85 kd subunit (p85α) lacks PI3-kinase activity and acts as an adaptor, coupling the 110 kd subunit (p110) to activated protein tyrosine kinases. Here the characterization of the p110 subunit is presented. cDNA cloning reveals p110 to be a 1068 aa protein related to Vps34p, a S. cerevisiae protein involved in the sorting of proteins to the vacuole. p110 expressed in insect cells possesses PI3-kinase activity and associates with p85α into an active p85α-p110 complex that binds the activated colony-stimulating factor 1 receptor. p110 expressed in COS-1 cells is catalytically active only when complexed with p85α.",
author = "Hiles, {Ian D.} and Masayuki Otsu and Stefano Volinia and Fry, {Michael J.} and Ivan Gout and Ritu Dhand and George Panayotou and Fernanda Ruiz-Larrea and Andrew Thompson and Totty, {Nicholas F.} and Hsuan, {J. Justin} and Courtneidge, {Sara A.} and Parker, {Peter J.} and Waterfield, {Michael D.}",
note = "Funding Information: We would like to thank the following people for help and materials provided during the course of this work: Dr. G. St. Whitley and Dr. A. Johnstone (St. Georges Hospital, London) for the SGBAF-1 cell line, Dr. M. Page (Wellcome Biotech, Beckenham, Kent) for the p36C baculovirus transfer vector, M. Jones (EMBL, Heidelberg) for help in the preparation of the ~110 C-terminal peptide antiserum, and Dr. C. Sherr (Howard Hughes Medical Institute, University of Tennessee, Memphis, Tennessee) for the CSF-1 receptor antiserum and cDNA. Finally, we would like to thank Mr. G. Scrace for help with DNA and protein sequence analysis, Mr. A. Sterling for oligonucleotide synthesis, and Dr. S. Emr for helpful discussions. I. G. was supported by a grant from Imperial Chemical Industries during part of his work on this project.",
year = "1992",
month = aug,
day = "7",
doi = "10.1016/0092-8674(92)90166-A",
language = "English (US)",
volume = "70",
pages = "419--429",
journal = "Cell",
issn = "0092-8674",
publisher = "Cell Press",
number = "3",
}