@inbook{d8279ce248474913b89c50e5410572e2,
title = "Photo-releasable derivatives of inositol pyrophosphates",
abstract = "The specific non-invasive control of intracellular signaling events requires advanced tools that enter cells by diffusion and are controllable by light. Here, we detail the synthesis and application of membrane-permeant caged inositol pyrophosphates with respect to cell entry and cell distribution. We recently published the synthesis of these tools as well as their effect on PH-domain localization in HeLa cells and oscillations of the intracellular calcium concentration in β-cells, which are known to drive insulin secretion. In this chapter, we discuss the possibilities and limitations when using cell-penetrating inositol pyrophosphates. We provide a detailed protocol for the application in live mouse β-cells and we discuss the image analysis needed for following effects on calcium signaling.",
keywords = "Cell entry, Inositol phosphate, Photo-activation, Prodrugs, Signaling",
author = "Sebastian Hauke and Tamara Bittner and Jessen, {Henning J.} and Carsten Schultz",
note = "Publisher Copyright: {\textcopyright} 2020 Elsevier Inc.",
year = "2020",
doi = "10.1016/bs.mie.2020.04.036",
language = "English (US)",
isbn = "9780128211564",
series = "Methods in Enzymology",
publisher = "Academic Press Inc.",
pages = "53--73",
editor = "Chenoweth, {David M.}",
booktitle = "Chemical Tools for Imaging, Manipulating, and Tracking Biological Systems",
}