Photoaffinity labeling of dog pancreas microsomes with 8-azido-ATP inhibits association of nascent preprolactin with the signal sequence receptor complex

Richard Zimmermann; Maria Zimmermann; Peter Mayinger; Peter Klappa

doi:10.1016/0014-5793(91)80949-4

Photoaffinity labeling of dog pancreas microsomes with 8-azido-ATP inhibits association of nascent preprolactin with the signal sequence receptor complex

Richard Zimmermann
, Maria Zimmermann
, Peter Mayinger
, Peter Klappa

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Transport of bovine preprolactin into dog pancreas microsomes involves a microsomal protein which is sensitive to photoaffinity labeling with azido-ATP and which is distinct from the ATP-binding protein, immunoglobulin heavy chain binding protein. Here we addressed the question of what stage of preprolactin transport is affected. Thus a nascent presecretory protein which is related to preprolactin, termed ppl-86mer, was employed. Here we show that the nascent preprolactin did not become associated with the α-subunit of the signal sequence receptor complex after photoaffinity labeling of microsomes with azido-ATP. Therefore, we conclude that the microsomal protein which is sensitive to photoaffinity labeling with azido-ATP acts prior to the signal sequence receptor complex.

Original language	English (US)
Pages (from-to)	95-99
Number of pages	5
Journal	FEBS Letters
Volume	286
Issue number	1-2
DOIs	https://doi.org/10.1016/0014-5793(91)80949-4
State	Published - Jul 29 1991
Externally published	Yes

Funding

Ackrto~vled8enrenu: WC thank Dr. Peter Walter for plasmid pSPB4 and Drs. Tom liapoport and Martin Wicdmonn for making available to US the protocol for chemical cross-linking prior to publication. This work was supported by the Sondcrforschungsbcreich I84 (Grant BIU), by the Sonderforschungsbcrcich 236 (Grant B22), and by the Fends clcr Chcmischcn Industric.

Funders
Fends clcr Chcmischcn Industric
Sondcrforschungsbcreich I84
Sonderforschungsbcrcich 236

Keywords

Endoplasmic reticulum
Protein transport

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(91)80949-4

Cite this

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title = "Photoaffinity labeling of dog pancreas microsomes with 8-azido-ATP inhibits association of nascent preprolactin with the signal sequence receptor complex",

abstract = "Transport of bovine preprolactin into dog pancreas microsomes involves a microsomal protein which is sensitive to photoaffinity labeling with azido-ATP and which is distinct from the ATP-binding protein, immunoglobulin heavy chain binding protein. Here we addressed the question of what stage of preprolactin transport is affected. Thus a nascent presecretory protein which is related to preprolactin, termed ppl-86mer, was employed. Here we show that the nascent preprolactin did not become associated with the α-subunit of the signal sequence receptor complex after photoaffinity labeling of microsomes with azido-ATP. Therefore, we conclude that the microsomal protein which is sensitive to photoaffinity labeling with azido-ATP acts prior to the signal sequence receptor complex.",

keywords = "Endoplasmic reticulum, Protein transport",

author = "Richard Zimmermann and Maria Zimmermann and Peter Mayinger and Peter Klappa",

note = "Funding Information: Ackrto\textasciitilde{}vled8enrenu: WC thank Dr. Peter Walter for plasmid pSPB4 and Drs. Tom liapoport and Martin Wicdmonn for making available to US the protocol for chemical cross-linking prior to publication. This work was supported by the Sondcrforschungsbcreich I84 (Grant BIU), by the Sonderforschungsbcrcich 236 (Grant B22), and by the Fends clcr Chcmischcn Industric.",

year = "1991",

month = jul,

day = "29",

doi = "10.1016/0014-5793(91)80949-4",

language = "English (US)",

volume = "286",

pages = "95--99",

journal = "FEBS Letters",

issn = "0014-5793",

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number = "1-2",

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TY - JOUR

T1 - Photoaffinity labeling of dog pancreas microsomes with 8-azido-ATP inhibits association of nascent preprolactin with the signal sequence receptor complex

AU - Zimmermann, Richard

AU - Zimmermann, Maria

AU - Mayinger, Peter

AU - Klappa, Peter

N1 - Funding Information: Ackrto~vled8enrenu: WC thank Dr. Peter Walter for plasmid pSPB4 and Drs. Tom liapoport and Martin Wicdmonn for making available to US the protocol for chemical cross-linking prior to publication. This work was supported by the Sondcrforschungsbcreich I84 (Grant BIU), by the Sonderforschungsbcrcich 236 (Grant B22), and by the Fends clcr Chcmischcn Industric.

PY - 1991/7/29

Y1 - 1991/7/29

N2 - Transport of bovine preprolactin into dog pancreas microsomes involves a microsomal protein which is sensitive to photoaffinity labeling with azido-ATP and which is distinct from the ATP-binding protein, immunoglobulin heavy chain binding protein. Here we addressed the question of what stage of preprolactin transport is affected. Thus a nascent presecretory protein which is related to preprolactin, termed ppl-86mer, was employed. Here we show that the nascent preprolactin did not become associated with the α-subunit of the signal sequence receptor complex after photoaffinity labeling of microsomes with azido-ATP. Therefore, we conclude that the microsomal protein which is sensitive to photoaffinity labeling with azido-ATP acts prior to the signal sequence receptor complex.

AB - Transport of bovine preprolactin into dog pancreas microsomes involves a microsomal protein which is sensitive to photoaffinity labeling with azido-ATP and which is distinct from the ATP-binding protein, immunoglobulin heavy chain binding protein. Here we addressed the question of what stage of preprolactin transport is affected. Thus a nascent presecretory protein which is related to preprolactin, termed ppl-86mer, was employed. Here we show that the nascent preprolactin did not become associated with the α-subunit of the signal sequence receptor complex after photoaffinity labeling of microsomes with azido-ATP. Therefore, we conclude that the microsomal protein which is sensitive to photoaffinity labeling with azido-ATP acts prior to the signal sequence receptor complex.

KW - Endoplasmic reticulum

KW - Protein transport

UR - https://www.scopus.com/pages/publications/0025916539

UR - https://www.scopus.com/pages/publications/0025916539#tab=citedBy

U2 - 10.1016/0014-5793(91)80949-4

DO - 10.1016/0014-5793(91)80949-4

M3 - Article

C2 - 1864386

AN - SCOPUS:0025916539

SN - 0014-5793

VL - 286

SP - 95

EP - 99

JO - FEBS Letters

JF - FEBS Letters

IS - 1-2

ER -

Photoaffinity labeling of dog pancreas microsomes with 8-azido-ATP inhibits association of nascent preprolactin with the signal sequence receptor complex

Abstract

Funding

Keywords

ASJC Scopus subject areas

Access to Document

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