Preliminary Results on Phosphorus-31 Nuclear Magnetic Resonance Evaluation of Human Uveal Melanoma in Enucleated Eyes

Nancy H. Kolodny; Evangelos S. Gragoudas; Donald J. D'Amico; Johanna M. Seddon; Margaret Minichiello; Elizabeth J. Murphy; Daniel M. Albert

doi:10.1016/S0161-6420(88)33129-5

Preliminary Results on Phosphorus-31 Nuclear Magnetic Resonance Evaluation of Human Uveal Melanoma in Enucleated Eyes

Nancy H. Kolodny, Evangelos S. Gragoudas, Donald J. D'Amico, Johanna M. Seddon, Margaret Minichiello, Elizabeth J. Murphy, Daniel M. Albert

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Clinical evaluation of uveal melanomas by nuclear magnetic resonance (NMR) techniques depends on ascertaining how these tumors characteristically appear in NMR images and spectra. The authors have determined NMR characteristics of suspected uveal melanomas by phosphorus-31 (³¹P) NMR spectroscopy of freshly enucleated human eyes. Nuclear magnetic resonance examination was performed at 8.45 Tesla within 90 minutes after enucleation. Enucleated eyes were maintained at 4°C in tissue culture medium during the 30 minutes required for transport. Nuclear magnetic resonance spectra were obtained within 10 minutes, a clinically acceptable time, using a two-turn ³¹P surface coil. Spectral parameters included 10-kHz spectral width, 1024 data points, and 0.5-second recycle delay. Phosphorus-31 NMR spectroscopy allowed differentiation of choroidal melanomas from normal ocular structures. Differentiating features include significant peaks in tumor spectra due to the phosphodiesters glycerol 3-phosphoryl ethanolamine (GPE) and glycerol 3phosphorylcholine (GPC), and the phosphomonoesters phosphorylethanolamine (PE)and phosphorylcholine (PC). These preliminary data are encouraging and suggest that clinical trials at the lower magnetic field strengths available in NMR imaging systems seem feasible and warrant investigation.

Original language	English (US)
Pages (from-to)	666-673
Number of pages	8
Journal	Ophthalmology
Volume	95
Issue number	5
DOIs	https://doi.org/10.1016/S0161-6420(88)33129-5
State	Published - 1988
Externally published	Yes

ASJC Scopus subject areas

Ophthalmology

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10.1016/S0161-6420(88)33129-5

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@article{7dfd4b3f1c11429f93d0697cd575a1c5,

title = "Preliminary Results on Phosphorus-31 Nuclear Magnetic Resonance Evaluation of Human Uveal Melanoma in Enucleated Eyes",

abstract = "Clinical evaluation of uveal melanomas by nuclear magnetic resonance (NMR) techniques depends on ascertaining how these tumors characteristically appear in NMR images and spectra. The authors have determined NMR characteristics of suspected uveal melanomas by phosphorus-31 (31P) NMR spectroscopy of freshly enucleated human eyes. Nuclear magnetic resonance examination was performed at 8.45 Tesla within 90 minutes after enucleation. Enucleated eyes were maintained at 4°C in tissue culture medium during the 30 minutes required for transport. Nuclear magnetic resonance spectra were obtained within 10 minutes, a clinically acceptable time, using a two-turn 31P surface coil. Spectral parameters included 10-kHz spectral width, 1024 data points, and 0.5-second recycle delay. Phosphorus-31 NMR spectroscopy allowed differentiation of choroidal melanomas from normal ocular structures. Differentiating features include significant peaks in tumor spectra due to the phosphodiesters glycerol 3-phosphoryl ethanolamine (GPE) and glycerol 3phosphorylcholine (GPC), and the phosphomonoesters phosphorylethanolamine (PE)and phosphorylcholine (PC). These preliminary data are encouraging and suggest that clinical trials at the lower magnetic field strengths available in NMR imaging systems seem feasible and warrant investigation.",

author = "Kolodny, {Nancy H.} and Gragoudas, {Evangelos S.} and D'Amico, {Donald J.} and Seddon, {Johanna M.} and Margaret Minichiello and Murphy, {Elizabeth J.} and Albert, {Daniel M.}",

note = "Funding Information: Supported in part by the Lions Eye Research Fund (Drs. Kolodny, D'Amico, and Albert), Research to Prevent Blindness and grant no. EY01917 (Drs. Albert and Kolodny). The NMR Resource of the Francis Bitter National Magnet Laboratory is supported by grant no. RR0095 of the Division of Research Resources of the National Institutes of Health and by National Science Foundation contract C-670.",

year = "1988",

doi = "10.1016/S0161-6420(88)33129-5",

language = "English (US)",

volume = "95",

pages = "666--673",

journal = "Ophthalmology",

issn = "0161-6420",

publisher = "Elsevier Inc.",

number = "5",

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TY - JOUR

T1 - Preliminary Results on Phosphorus-31 Nuclear Magnetic Resonance Evaluation of Human Uveal Melanoma in Enucleated Eyes

AU - Kolodny, Nancy H.

AU - Gragoudas, Evangelos S.

AU - D'Amico, Donald J.

AU - Seddon, Johanna M.

AU - Minichiello, Margaret

AU - Murphy, Elizabeth J.

AU - Albert, Daniel M.

N1 - Funding Information: Supported in part by the Lions Eye Research Fund (Drs. Kolodny, D'Amico, and Albert), Research to Prevent Blindness and grant no. EY01917 (Drs. Albert and Kolodny). The NMR Resource of the Francis Bitter National Magnet Laboratory is supported by grant no. RR0095 of the Division of Research Resources of the National Institutes of Health and by National Science Foundation contract C-670.

PY - 1988

Y1 - 1988

N2 - Clinical evaluation of uveal melanomas by nuclear magnetic resonance (NMR) techniques depends on ascertaining how these tumors characteristically appear in NMR images and spectra. The authors have determined NMR characteristics of suspected uveal melanomas by phosphorus-31 (31P) NMR spectroscopy of freshly enucleated human eyes. Nuclear magnetic resonance examination was performed at 8.45 Tesla within 90 minutes after enucleation. Enucleated eyes were maintained at 4°C in tissue culture medium during the 30 minutes required for transport. Nuclear magnetic resonance spectra were obtained within 10 minutes, a clinically acceptable time, using a two-turn 31P surface coil. Spectral parameters included 10-kHz spectral width, 1024 data points, and 0.5-second recycle delay. Phosphorus-31 NMR spectroscopy allowed differentiation of choroidal melanomas from normal ocular structures. Differentiating features include significant peaks in tumor spectra due to the phosphodiesters glycerol 3-phosphoryl ethanolamine (GPE) and glycerol 3phosphorylcholine (GPC), and the phosphomonoesters phosphorylethanolamine (PE)and phosphorylcholine (PC). These preliminary data are encouraging and suggest that clinical trials at the lower magnetic field strengths available in NMR imaging systems seem feasible and warrant investigation.

AB - Clinical evaluation of uveal melanomas by nuclear magnetic resonance (NMR) techniques depends on ascertaining how these tumors characteristically appear in NMR images and spectra. The authors have determined NMR characteristics of suspected uveal melanomas by phosphorus-31 (31P) NMR spectroscopy of freshly enucleated human eyes. Nuclear magnetic resonance examination was performed at 8.45 Tesla within 90 minutes after enucleation. Enucleated eyes were maintained at 4°C in tissue culture medium during the 30 minutes required for transport. Nuclear magnetic resonance spectra were obtained within 10 minutes, a clinically acceptable time, using a two-turn 31P surface coil. Spectral parameters included 10-kHz spectral width, 1024 data points, and 0.5-second recycle delay. Phosphorus-31 NMR spectroscopy allowed differentiation of choroidal melanomas from normal ocular structures. Differentiating features include significant peaks in tumor spectra due to the phosphodiesters glycerol 3-phosphoryl ethanolamine (GPE) and glycerol 3phosphorylcholine (GPC), and the phosphomonoesters phosphorylethanolamine (PE)and phosphorylcholine (PC). These preliminary data are encouraging and suggest that clinical trials at the lower magnetic field strengths available in NMR imaging systems seem feasible and warrant investigation.

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ER -

Preliminary Results on Phosphorus-31 Nuclear Magnetic Resonance Evaluation of Human Uveal Melanoma in Enucleated Eyes

Abstract

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