TY - JOUR
T1 - Prenatal valproic acid exposure decreases neuronal membrane order in rat offspring hippocampus and cortex
AU - Vorhees, Charles V.
AU - Rauch, Scott L.
AU - Hitzemann, Robert J.
N1 - Funding Information:
The authors wish to acknowledge the research assistance of C. A. Blanton and M. S. Moran. This work was supported by PHS grant AA6032 from the National Institute on Alcohol Abuse and Alcoholism.
PY - 1991
Y1 - 1991
N2 - Sprague-Dawley rats were administered 0 or 200 mg/kg of valproic acid (VPA) on days 7-18 of gestation. Controls were pair-fed. On postnatal day 28, analyses of brain tissues were performed on enriched neuronal membrane fractions from cerebellum, hippocampus, and cortex for anisotropy using the membrane probe 1,6-diphenyl-1,3,5-hexatriene (DPH). No significant differences in membrane anisotropy were noted in the cerebellum, but a significant reduction in anisotropy in the hippocampus and cortex was observed. This change corresponds to a 3-5°C increase in temperature or that produced by other membrane disordering agents (ethanol, phenytoin). An association between membrane anisotropy and functional effects of prenatal VPA exposure is discussed.
AB - Sprague-Dawley rats were administered 0 or 200 mg/kg of valproic acid (VPA) on days 7-18 of gestation. Controls were pair-fed. On postnatal day 28, analyses of brain tissues were performed on enriched neuronal membrane fractions from cerebellum, hippocampus, and cortex for anisotropy using the membrane probe 1,6-diphenyl-1,3,5-hexatriene (DPH). No significant differences in membrane anisotropy were noted in the cerebellum, but a significant reduction in anisotropy in the hippocampus and cortex was observed. This change corresponds to a 3-5°C increase in temperature or that produced by other membrane disordering agents (ethanol, phenytoin). An association between membrane anisotropy and functional effects of prenatal VPA exposure is discussed.
KW - Anisotropy in cortex
KW - Anisotropy in hippocampus
KW - In utero valproic acid and membrane anisotropy
KW - Prenatal valproic acid
KW - Rats
KW - Synaptic membrane order
KW - Valproate-induced reduction in membrane order
UR - http://www.scopus.com/inward/record.url?scp=0025848027&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025848027&partnerID=8YFLogxK
U2 - 10.1016/0892-0362(91)90097-G
DO - 10.1016/0892-0362(91)90097-G
M3 - Article
C2 - 1921927
AN - SCOPUS:0025848027
SN - 0892-0362
VL - 13
SP - 471
EP - 474
JO - Neurotoxicology and Teratology
JF - Neurotoxicology and Teratology
IS - 4
ER -