TY - JOUR
T1 - Primary structure of the gene encoding rat preprosomatostatin
AU - Montminy, M. R.
AU - Goodman, R. H.
AU - Horovitch, S. J.
AU - Habener, J. F.
PY - 1984
Y1 - 1984
N2 - The somatostatins are peptides of 14 and 28 amino acids that are produced in a variety of endocrine and nonendocrine tissues. These peptides inhibit the secretion of many different pituitary, pancreatic, and gastrointestinal hormones. Previously, we have reported the isolation and nucleotide sequence of a cDNA derived from a rat medullary thyroid carcinoma that encoded preprosomatostatin, a 116-amino-acid precursor of somatostatin. We now report the structural characterization of the rat somatostatin gene isolated from recombinant bacteriophage libraries prepared from rat liver DNA. The gene spans 1.2 kilobases and is interrupted within the coding sequence of prosomatostatin by a single intron of 630 bases. A sequence characteristic of a Goldberg-Hogness promoter ('TATA' box), T-T-T-A-A-A-A, is located 31 bases up-stream from the transcriptional initiation site. A repetitive DNA sequence, highly reiterated in the rat genome, is located in the 5' flanking region of the gene within 900 bases of the initiation site.
AB - The somatostatins are peptides of 14 and 28 amino acids that are produced in a variety of endocrine and nonendocrine tissues. These peptides inhibit the secretion of many different pituitary, pancreatic, and gastrointestinal hormones. Previously, we have reported the isolation and nucleotide sequence of a cDNA derived from a rat medullary thyroid carcinoma that encoded preprosomatostatin, a 116-amino-acid precursor of somatostatin. We now report the structural characterization of the rat somatostatin gene isolated from recombinant bacteriophage libraries prepared from rat liver DNA. The gene spans 1.2 kilobases and is interrupted within the coding sequence of prosomatostatin by a single intron of 630 bases. A sequence characteristic of a Goldberg-Hogness promoter ('TATA' box), T-T-T-A-A-A-A, is located 31 bases up-stream from the transcriptional initiation site. A repetitive DNA sequence, highly reiterated in the rat genome, is located in the 5' flanking region of the gene within 900 bases of the initiation site.
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U2 - 10.1073/pnas.81.11.3337
DO - 10.1073/pnas.81.11.3337
M3 - Article
C2 - 6145156
AN - SCOPUS:0021278563
SN - 0027-8424
VL - 81
SP - 3337
EP - 3340
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 11 I
ER -