Profiling Esterases in Mycobacterium tuberculosis Using Far-Red Fluorogenic Substrates

Katie R. Tallman, Samantha R. Levine, Kimberly E. Beatty

Research output: Contribution to journalArticlepeer-review

30 Scopus citations


Enzyme-activated, fluorogenic probes are powerful tools for studying bacterial pathogens, including Mycobacterium tuberculosis (Mtb). In prior work, we reported two 7-hydroxy-9H-(1,3-dichloro-9,9-dimethylacridin-2-one) (DDAO)-derived acetoxymethyl ether probes for esterase and lipase detection. Here, we report four-carbon (C4) and eight-carbon (C8) acyloxymethyl ether derivatives, which are longer-chain fluorogenic substrates. These new probes demonstrate greater stability and lipase reactivity than the two-carbon (C2) acetoxymethyl ether-masked substrates. We used these new C4 and C8 probes to profile esterases and lipases from Mtb. The C8-masked probes revealed a new esterase band in gel-resolved Mtb lysates that was not present in lysates from nonpathogenic M. bovis (bacillus Calmette-Guérin), a close genetic relative. We identified this Mtb-specific enzyme as the secreted esterase Culp1 (Rv1984c). Our C4- and C8-masked probes also produced distinct Mtb banding patterns in lysates from Mtb-infected macrophages, demonstrating the potential of these probes for detecting Mtb esterases that are active during infections.

Original languageEnglish (US)
Pages (from-to)1810-1815
Number of pages6
JournalACS chemical biology
Issue number7
StatePublished - Jul 15 2016

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine


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