Progesterone 5α-reductase in mouse brain

Charles E. Roselli, Charles A. Snipes

Research output: Contribution to journalArticlepeer-review

4 Scopus citations


We have examined the distribution of progesterone 5α-reductase activity in minces of discrete brain areas from two strains of adult ovariectomized mice known to be differentially responsive to the behavioral effects of exogenously administered 5α-pregnane-3,20-dione (5α-DHP). Minces of freshly dissected midbrain tegmentum (TEG), hypothalamus-preoptic area (HPOA), hippocampus (HIP), and parietal cortex (CTX) were incubated with [4-14C]progesterone in a Krebs-Henseleit bicarbonate buffer solution containing 10 mM glucose for 2 h at 37°C. The [14C]5α-DHP produced was identified by comparison with known standards in paper and thin-layer chromatography systems, derivative formation, and recrystallization to a constant 14C/3H ratio. We found a 6-fold difference in the extent to which various brain areas formed 5α-DHP. The amount of conversion was similar for both strains of mice. When expressed as picomoles of 5α-DHP formed per 2 h per 20 mg of tissue, the following order of activity was observed: TEG > HPOA > HIP > CTX.

Original languageEnglish (US)
Pages (from-to)197-202
Number of pages6
JournalBrain research
Issue number2
StatePublished - Jul 9 1984
Externally publishedYes


  • 5α-dihydroprogesterone
  • 5α-reductase
  • mouse brain
  • progesterone metabolism

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology


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