@article{8ecfd9952acc463fa658edf30e95cd7d,
title = "Promotion of fibronectin independent invasion by C5a peptidase into epithelial cells in group A Streptococcus",
abstract = "Background \& objectives: Group A Streptococcus, causative agent of several clinical manifestations codes for multiple protein invasins which help the bacterium to enter non-phagocytic cells. C5a peptidase (SCPA) is a surface protein conserved among different serotypes of M1 strain. The present study was taken up to study SCPA promoted fibronectin independent entry of GAS into epithelial cells. Methods: An isogenic 90226 emm1ΔAB (M1-) mutant was constructed with thermosensitive pGhost vector. This isogenic M1- mutant expressed SCPA on the surface as determined by Western blotting and immunofluorescence. Results: On preincubation with anti-SCPA serum, the isogenic M1- strain exhibited 54 per cent decreased invasion as compared to the bacteria incubated with control serum. Also, purified recombinant SCPA proteins blocked internalization of M1- streptococci into HEp-2 cells. The M1- strain invaded at the same efficiency in the presence or absence of fibronectin. Interpretation \& conclusion: These results suggested that SCPA acted as a potential invasin of group A streptococcus and promoted invasion independent of fibronectin.",
keywords = "C5a peptidase, Fibronectin, Group A streptococcus, Invasion, M1 strain",
author = "Purushothaman, \{Sai Sudha\} and Park, \{Hae Sun\} and Cleary, \{P. Patrick\}",
year = "2004",
language = "English (US)",
volume = "119",
pages = "44--47",
journal = "Indian Journal of Medical Research, Supplement",
issn = "0367-9012",
publisher = "Wolters Kluwer Health",
number = "MAY",
}