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Prostratin exhibits both replication enhancing and inhibiting effects on FIV infection of feline CD4+ T-cells

  • Chi Ngai Chan
  • , Elizabeth L. McMonagle
  • , Margaret J. Hosie
  • , Brian J. Willett

Research output: Contribution to journalArticlepeer-review

Abstract

The phorbol ester Prostratin may either stimulate or inhibit human immunodeficiency virus-1 (HIV-1) replication. Here we report that Prostratin also exhibits a similar dual action upon feline immunodeficiency virus (FIV) replication in an IL-2-dependent feline CD4+ T-cell line (MYA-1). While withdrawal of IL-2 halted FIV spread, Prostratin rescued virus production and cell viability, mimicking the functions of the cytokine. Conversely, FIV grew rapidly in the presence of IL-2 and this was inhibited by Prostratin. In contrast to HIV-1, Prostratin mediated inhibition of FIV through means other than blocking virus entry. Co-application of the protein kinase C (PKC) inhibitor Gö6850 with Prostratin reversed both the inhibitory and stimulatory effects, suggesting that PKC is crucial for FIV replication.

Original languageEnglish (US)
Pages (from-to)121-128
Number of pages8
JournalVirus Research
Volume171
Issue number1
DOIs
StatePublished - Jan 2013
Externally publishedYes

Funding

FundersFunder number
Medical Research Council/Biological Sciences Research CouncilG0801822, G0300387
Biotechnology and Biological Sciences Research CouncilBB/D008425/1

    Keywords

    • CD4 T-cells
    • FIV
    • HIV-1
    • IL-2
    • PKC
    • PMA
    • Prostratin
    • Protein kinase C

    ASJC Scopus subject areas

    • Infectious Diseases
    • Cancer Research
    • Virology

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