TY - JOUR
T1 - Protein binding to the interferon response enhancer correlates with interferon induction of 2′-5′-oligoadenylate synthetase in normal and interferon-resistant Friend cells
AU - Coccia, Eliana M.
AU - Vaiman, Daniel
AU - Raber, Jacob
AU - Marziali, Giovanna
AU - Fiorucci, Gianna
AU - Orsatti, Roberto
AU - Cohen, Batya
AU - Nissim, Nili
AU - Romeo, Giovanna
AU - Abris, Elisabetta Aff
AU - Chebath, Judith
AU - Battistini, Angela
PY - 1991
Y1 - 1991
N2 - The induction of transcription of the 2prime;-5prime-oIigoadenylate (2-5A) synthetase gene by type I (α/β) and type II (γ) interferons (IFNs) has been studied in wild-type (w.t.) and IFN-resistant Friend leukemia cells (FLC). Following IFN treatment, new complexes are formed in vitro between the IFN-responsive sequence (IRS) of the 2-5A synthetase gene and cellular proteins. Within minutes after IFN-α/β addition to w.t. FLC, an IRS-protein complex, designated F1, is detected, as already observed in several human cell lines. In response to IFN-γ, a novel complex, designated Fg, is observed in w.t. FLC. The Fg complex appears within 3 h, while an F1-like complex is faintly visible 10 to 24 h later. In the IFN-α/β-resistant FLC, IFN-γ induces only the Fg complex and fails to induce F1. Fg formation is correlated with the IFN-γ-induced transcription of the 2-5A synthetase gene and the appearance of the corresponding enzymatic activity in both w.t. and IFN-α/β-resistant FLC. These findings suggest that F1 and Fg represent two distinct effector complexes by which type I and type II IFNs, respectively, induce 2-5A synthetase.
AB - The induction of transcription of the 2prime;-5prime-oIigoadenylate (2-5A) synthetase gene by type I (α/β) and type II (γ) interferons (IFNs) has been studied in wild-type (w.t.) and IFN-resistant Friend leukemia cells (FLC). Following IFN treatment, new complexes are formed in vitro between the IFN-responsive sequence (IRS) of the 2-5A synthetase gene and cellular proteins. Within minutes after IFN-α/β addition to w.t. FLC, an IRS-protein complex, designated F1, is detected, as already observed in several human cell lines. In response to IFN-γ, a novel complex, designated Fg, is observed in w.t. FLC. The Fg complex appears within 3 h, while an F1-like complex is faintly visible 10 to 24 h later. In the IFN-α/β-resistant FLC, IFN-γ induces only the Fg complex and fails to induce F1. Fg formation is correlated with the IFN-γ-induced transcription of the 2-5A synthetase gene and the appearance of the corresponding enzymatic activity in both w.t. and IFN-α/β-resistant FLC. These findings suggest that F1 and Fg represent two distinct effector complexes by which type I and type II IFNs, respectively, induce 2-5A synthetase.
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M3 - Article
C2 - 1705997
AN - SCOPUS:0026057920
SN - 0022-538X
VL - 65
SP - 2081
EP - 2087
JO - Journal of virology
JF - Journal of virology
IS - 4
ER -