TY - JOUR
T1 - Protein synthesis and the presence or absence of a measurable G1 in cultured chinese hamster cells
AU - Liskay, R. Michael
AU - Kornfeld, Bruce
AU - Fullerton, Paul
AU - Evans, Ronald
PY - 1980/9
Y1 - 1980/9
N2 - V79‐8 cells lack a measurable G1 interval under normal growth conditions. We found that partial inhibition of protein synthesis using low levels of cycloheximide (0.05 μ/ml) could induce a measurable G1 in these cells without any significant effects on S, G2, or M. In view of these findings, recessive mutants selected from the V79‐8 cell line, which each express G1, were analyzed for their rates of protein synthesis and degradation/loss. Three of the four mutants showed a decreased rate of protein synthesis sufficient to account for their G1 lengths. A fourth mutant, however, showed parental rates of both protein synthesis and degradation/loss. These results suggest not only that a G1 interval can be expressed as a result of a decreased rate of protein synthesis, but that other alterations (mutations) other than those simply affecting overall protein synthesis can result in the expression of a measureable G1 interval.
AB - V79‐8 cells lack a measurable G1 interval under normal growth conditions. We found that partial inhibition of protein synthesis using low levels of cycloheximide (0.05 μ/ml) could induce a measurable G1 in these cells without any significant effects on S, G2, or M. In view of these findings, recessive mutants selected from the V79‐8 cell line, which each express G1, were analyzed for their rates of protein synthesis and degradation/loss. Three of the four mutants showed a decreased rate of protein synthesis sufficient to account for their G1 lengths. A fourth mutant, however, showed parental rates of both protein synthesis and degradation/loss. These results suggest not only that a G1 interval can be expressed as a result of a decreased rate of protein synthesis, but that other alterations (mutations) other than those simply affecting overall protein synthesis can result in the expression of a measureable G1 interval.
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U2 - 10.1002/jcp.1041040318
DO - 10.1002/jcp.1041040318
M3 - Article
C2 - 7419616
AN - SCOPUS:0019254426
SN - 0021-9541
VL - 104
SP - 461
EP - 467
JO - Journal of Cellular Physiology
JF - Journal of Cellular Physiology
IS - 3
ER -