Purified hematopoietic stem cells can differentiate into hepatocytes in vivo

Eric Lagasse; Heather Connors; Muhsen Al-Dhalimy; Michael Reitsma; Monika Dohse; Linda Osborne; Xin Wang; Milton Finegold; Irving L. Weissman; Markus Grompe

doi:10.1038/81326

Purified hematopoietic stem cells can differentiate into hepatocytes in vivo

Eric Lagasse, Heather Connors, Muhsen Al-Dhalimy, Michael Reitsma, Monika Dohse, Linda Osborne, Xin Wang, Milton Finegold, Irving L. Weissman, Markus Grompe

Pediatrics

Research output: Contribution to journal › Article › peer-review

2173 Scopus citations

Abstract

The characterization of hepatic progenitor cells is of great scientific and clinical interest. Here we report that intravenous injection of adult bone marrow cells in the FAH(-/-) mouse, an animal model of tyrosinemia type I, rescued the mouse and restored the biochemical function of its liver. Moreover, within bone marrow, only rigorously purified hematopoietic stem cells gave rise to donor-derived hematopoietic and hepatic regeneration. This result seems to contradict the conventional assumptions of the germ layer origins of tissues such as the liver, and raises the question of whether the cells of the hematopoietic stem cell phenotype are pluripotent hematopoietic cells that retain the ability to transdifferentiate, or whether they are more primitive multipotent cells.

Original language	English (US)
Pages (from-to)	1229-1234
Number of pages	6
Journal	Nature medicine
Volume	6
Issue number	11
DOIs	https://doi.org/10.1038/81326
State	Published - 2000

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology

Access to Document

10.1038/81326

Cite this

@article{ade244336d9348ae87b5b83ae334d1de,

title = "Purified hematopoietic stem cells can differentiate into hepatocytes in vivo",

abstract = "The characterization of hepatic progenitor cells is of great scientific and clinical interest. Here we report that intravenous injection of adult bone marrow cells in the FAH(-/-) mouse, an animal model of tyrosinemia type I, rescued the mouse and restored the biochemical function of its liver. Moreover, within bone marrow, only rigorously purified hematopoietic stem cells gave rise to donor-derived hematopoietic and hepatic regeneration. This result seems to contradict the conventional assumptions of the germ layer origins of tissues such as the liver, and raises the question of whether the cells of the hematopoietic stem cell phenotype are pluripotent hematopoietic cells that retain the ability to transdifferentiate, or whether they are more primitive multipotent cells.",

author = "Eric Lagasse and Heather Connors and Muhsen Al-Dhalimy and Michael Reitsma and Monika Dohse and Linda Osborne and Xin Wang and Milton Finegold and Weissman, {Irving L.} and Markus Grompe",

note = "Funding Information: Acknowledgments We thank L. Jerabek for help with the mice, A. Tsukamoto for review of the manuscript, M. Masek for optimizing staining protocols and M. Ferraz for animal care. This work was supported in part by the National Institutes of Health (I.L.W and M.G.) and the American Liver Foundation (X.W).",

year = "2000",

doi = "10.1038/81326",

language = "English (US)",

volume = "6",

pages = "1229--1234",

journal = "Nature medicine",

issn = "1078-8956",

publisher = "Nature Publishing Group",

number = "11",

}

TY - JOUR

T1 - Purified hematopoietic stem cells can differentiate into hepatocytes in vivo

AU - Lagasse, Eric

AU - Connors, Heather

AU - Al-Dhalimy, Muhsen

AU - Reitsma, Michael

AU - Dohse, Monika

AU - Osborne, Linda

AU - Wang, Xin

AU - Finegold, Milton

AU - Weissman, Irving L.

AU - Grompe, Markus

N1 - Funding Information: Acknowledgments We thank L. Jerabek for help with the mice, A. Tsukamoto for review of the manuscript, M. Masek for optimizing staining protocols and M. Ferraz for animal care. This work was supported in part by the National Institutes of Health (I.L.W and M.G.) and the American Liver Foundation (X.W).

PY - 2000

Y1 - 2000

N2 - The characterization of hepatic progenitor cells is of great scientific and clinical interest. Here we report that intravenous injection of adult bone marrow cells in the FAH(-/-) mouse, an animal model of tyrosinemia type I, rescued the mouse and restored the biochemical function of its liver. Moreover, within bone marrow, only rigorously purified hematopoietic stem cells gave rise to donor-derived hematopoietic and hepatic regeneration. This result seems to contradict the conventional assumptions of the germ layer origins of tissues such as the liver, and raises the question of whether the cells of the hematopoietic stem cell phenotype are pluripotent hematopoietic cells that retain the ability to transdifferentiate, or whether they are more primitive multipotent cells.

AB - The characterization of hepatic progenitor cells is of great scientific and clinical interest. Here we report that intravenous injection of adult bone marrow cells in the FAH(-/-) mouse, an animal model of tyrosinemia type I, rescued the mouse and restored the biochemical function of its liver. Moreover, within bone marrow, only rigorously purified hematopoietic stem cells gave rise to donor-derived hematopoietic and hepatic regeneration. This result seems to contradict the conventional assumptions of the germ layer origins of tissues such as the liver, and raises the question of whether the cells of the hematopoietic stem cell phenotype are pluripotent hematopoietic cells that retain the ability to transdifferentiate, or whether they are more primitive multipotent cells.

UR - http://www.scopus.com/inward/record.url?scp=0033694301&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033694301&partnerID=8YFLogxK

U2 - 10.1038/81326

DO - 10.1038/81326

M3 - Article

C2 - 11062533

AN - SCOPUS:0033694301

SN - 1078-8956

VL - 6

SP - 1229

EP - 1234

JO - Nature medicine

JF - Nature medicine

IS - 11

ER -

Purified hematopoietic stem cells can differentiate into hepatocytes in vivo

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this