Quantitative phase evaluation of dynamic changes on cell membrane during laser microsurgery

Lingfeng Yu, Samarendra Mohanty, Gangjun Liu, Suzanne Genc, Zhongping Chen, Michael W. Berns

Research output: Contribution to journalArticlepeer-review

29 Scopus citations


The ability to inject exogenous material as well as to alter subcellular structures in a minimally invasive manner using a laser microbeam has been useful for cell biologists to study the structure-function relationship in complex biological systems. We describe a quantitative phase laser microsurgery system, which takes advantage of the combination of laser microirradiation and short-coherence interference microscopy. Using this method, quantitative phase images and the dynamic changes of phase during the process of laser microsurgery of red blood cells (RBCs) can be evaluated in real time. This system would enable absolute quantitation of localized alteration/damage to transparent phase objects, such as the cell membrane or intracellular structures, being exposed to the laser microbeam. Such quantitation was not possible using conventional phase-contrast microscopy.

Original languageEnglish (US)
Article number050508
JournalJournal of biomedical optics
Issue number5
StatePublished - 2008
Externally publishedYes


  • cellular damage
  • laser scissors
  • optical micromanipulation
  • quantitative phase imaging
  • short-coherence interference microscopy

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering


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