@article{d56c79a16f2643c3b3a83a5be80ab93b,
title = "Rabbit P450 2E1 expressed in CHO-K1 cells has a short half-life",
abstract = "Rabbit P450 2E1 was stably expressed in Chinese hamster ovary cells after cotransfection with pRC/CMV-2E1 and pFR400 which expresses murine dihydrofolate reductase with a single arginine to leucine substitution at position 22. This mutation permits amplification of expression with increasing methotrexate concentrations in CHO-K1 cells that are not dihydrofolate reductase deficient. After amplification with 1 μM methotrexate, a representative clone expressed about 15 pmol of P450 2E1/mg microsomal protein. Cells from a single 35-mm plate catalyzed the formation of 1.02 nmol 6-hydroxychlorzoxazone/106 cells/h or about 127 pmol/mg total cell protein/min. The enzyme was rapidly labeled when pulsed with [35S]-methionine. Initial pulse-chase experiments indicate that the expressed protein has a half-life of 4.8 h.",
author = "Suzanne Barmada and Eric Kienle and Koop, \{Dennis R.\}",
year = "1995",
month = jan,
day = "17",
doi = "10.1006/bbrc.1995.1085",
language = "English (US)",
volume = "206",
pages = "601--607",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Elsevier B.V.",
number = "2",
}