Radioligand assay for Δ5-3β-hydroxysteroids I. 3β-hydroxy-5-androstene-17-one and its 3-sulfate

E. Nieschlag; D. L. Loriaux; M. B. Lipsett

doi:10.1016/S0039-128X(72)80032-1

Radioligand assay for Δ⁵-3β-hydroxysteroids I. 3β-hydroxy-5-androstene-17-one and its 3-sulfate

E. Nieschlag
, D. L. Loriaux
, M. B. Lipsett

Research output: Contribution to journal › Article › peer-review

60 Scopus citations

Abstract

A specific, sensitive and reliable radioligand assay for plasma dehydroepiandrosterone (DHA) and its sulfate has been developed. Antisera were obtained by immunizing rabbits with a DHA-17-albumin conjugate. DHA was separated from cross-reacting Δ⁵-steroids by thin layer chromatography. DHA-sulfate was solvolyzed prior to chromatography. Separation of antibody bound and free steroid was achieved with γ-globulin-dextran-coated charcoal. The standard curve was linear on a logit-log plot from 0.1 to 10 ng.

Original language	English (US)
Pages (from-to)	669-679
Number of pages	11
Journal	Steroids
Volume	19
Issue number	5
DOIs	https://doi.org/10.1016/S0039-128X(72)80032-1
State	Published - May 1972
Externally published	Yes

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Endocrinology
Pharmacology
Clinical Biochemistry
Organic Chemistry

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10.1016/S0039-128X(72)80032-1

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title = "Radioligand assay for Δ5-3β-hydroxysteroids I. 3β-hydroxy-5-androstene-17-one and its 3-sulfate",

abstract = "A specific, sensitive and reliable radioligand assay for plasma dehydroepiandrosterone (DHA) and its sulfate has been developed. Antisera were obtained by immunizing rabbits with a DHA-17-albumin conjugate. DHA was separated from cross-reacting Δ5-steroids by thin layer chromatography. DHA-sulfate was solvolyzed prior to chromatography. Separation of antibody bound and free steroid was achieved with γ-globulin-dextran-coated charcoal. The standard curve was linear on a logit-log plot from 0.1 to 10 ng.",

author = "E. Nieschlag and Loriaux, \{D. L.\} and Lipsett, \{M. B.\}",

year = "1972",

month = may,

doi = "10.1016/S0039-128X(72)80032-1",

language = "English (US)",

volume = "19",

pages = "669--679",

journal = "Steroids",

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AU - Loriaux, D. L.

AU - Lipsett, M. B.

PY - 1972/5

Y1 - 1972/5

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AB - A specific, sensitive and reliable radioligand assay for plasma dehydroepiandrosterone (DHA) and its sulfate has been developed. Antisera were obtained by immunizing rabbits with a DHA-17-albumin conjugate. DHA was separated from cross-reacting Δ5-steroids by thin layer chromatography. DHA-sulfate was solvolyzed prior to chromatography. Separation of antibody bound and free steroid was achieved with γ-globulin-dextran-coated charcoal. The standard curve was linear on a logit-log plot from 0.1 to 10 ng.

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JO - Steroids

JF - Steroids

IS - 5

ER -

Radioligand assay for Δ⁵-3β-hydroxysteroids I. 3β-hydroxy-5-androstene-17-one and its 3-sulfate

Abstract

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