@inproceedings{f93eff05d35447b6aefde9e3af295c58,
title = "Rapid detection of cancer related DNA nanoparticulate biomarkers and nanoparticles in whole blood",
abstract = "The ability to rapidly detect cell free circulating (cfc) DNA, cfc-RNA, exosomes and other nanoparticulate disease biomarkers as well as drug delivery nanoparticles directly in blood is a major challenge for nanomedicine. We now show that microarray and new high voltage dielectrophoretic (DEP) devices can be used to rapidly isolate and detect cfc-DNA nanoparticulates and nanoparticles directly from whole blood and other high conductance samples (plasma, serum, urine, etc.). At DEP frequencies of 5kHz-10kHz both fluorescent-stained high molecular weight (hmw) DNA, cfc-DNA and fluorescent nanoparticles separate from the blood and become highly concentrated at specific DEP highfield regions over the microelectrodes, while blood cells move to the DEP low field-regions. The blood cells can then be removed by a simple fluidic wash while the DNA and nanoparticles remain highly concentrated. The hmw-DNA could be detected at a level of <260ng/ml and the nanoparticles at <9.5 × 109 particles/ml, detection levels that are well within the range for viable clinical diagnostics and drug nanoparticle monitoring. Disease specific cfc-DNA materials could also be detected directly in blood from patients with Chronic Lymphocytic Leukemia (CLL) and confirmed by PCR genotyping analysis.",
keywords = "Biomarkers, Blood, Cancer diagnostics, Cfc-DNA, Dielectrophoresis (DEP), Exosomes, Nanoparticles, RNA",
author = "Heller, {Michael J.} and Raj Krishnan and Avery Sonnenberg",
year = "2010",
doi = "10.1117/12.861579",
language = "English (US)",
isbn = "9780819482556",
series = "Proceedings of SPIE - The International Society for Optical Engineering",
booktitle = "Biosensing III",
note = "Biosensing III ; Conference date: 01-08-2010 Through 03-08-2010",
}