Regional distribution of maternal messenger RNA in the amphibian oocyte

Mary Lou King; Eric Barklis

doi:10.1016/0012-1606(85)90134-4

Regional distribution of maternal messenger RNA in the amphibian oocyte

Mary Lou King
, Eric Barklis

Research output: Contribution to journal › Article › peer-review

52 Scopus citations

Abstract

We show that there is a non-random distribution of information in the form of mRNAs along the animal/vegetal axis of Xenopus oocytes. Polyadenylated RNA isolated from different regions along this axis was translated in a cell-free system, and the products were fractionated by two-dimensional gel electrophoresis. A comparison of the resulting protein products using a computer program has established that specific translatable mRNAs are regionally localized within the oocyte prior to fertilization. Seventeen mRNAs show significant qualitative or quantitative differences in their subcellular location. These sequences comprise as much as 4% of the total detected translatable polyadenylated RNA. Asymmetric segregation of these messages during Xenopus development may be involved in the determination of cell fate.

Original language	English (US)
Pages (from-to)	203-212
Number of pages	10
Journal	Developmental Biology
Volume	112
Issue number	1
DOIs	https://doi.org/10.1016/0012-1606(85)90134-4
State	Published - Nov 1985
Externally published	Yes

Funding

We thank Bob Power for expert technical assistance and unlimited patience in doing the cryostat sectioning. We acknowledge the valuable assistance of Luis Martarano in isolating the RNA. M.L.K. expresses her gratitude to Jim Garrison for his generosity in providing the computer program and for his help during the course of this study. We especially appreciate the support, comments, and enthusiasm Harvey Lodish has provided. This investigation was supported in part by grants from the National Science Foundation (PCM-8112215) and the March of Dimes (Basil O’Connor Research Award) to M.L.K.

Funders	Funder number
Author National Science Foundation National Science Foundation National Institutes of Health National Institutes of Health National Institutes of Health National Institutes of Health National Science Foundation National Science Foundation	PCM-8112215
March of Dimes Foundation

ASJC Scopus subject areas

Molecular Biology
Developmental Biology
Cell Biology

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10.1016/0012-1606(85)90134-4

Cite this

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title = "Regional distribution of maternal messenger RNA in the amphibian oocyte",

abstract = "We show that there is a non-random distribution of information in the form of mRNAs along the animal/vegetal axis of Xenopus oocytes. Polyadenylated RNA isolated from different regions along this axis was translated in a cell-free system, and the products were fractionated by two-dimensional gel electrophoresis. A comparison of the resulting protein products using a computer program has established that specific translatable mRNAs are regionally localized within the oocyte prior to fertilization. Seventeen mRNAs show significant qualitative or quantitative differences in their subcellular location. These sequences comprise as much as 4\% of the total detected translatable polyadenylated RNA. Asymmetric segregation of these messages during Xenopus development may be involved in the determination of cell fate.",

author = "King, \{Mary Lou\} and Eric Barklis",

note = "Funding Information: We thank Bob Power for expert technical assistance and unlimited patience in doing the cryostat sectioning. We acknowledge the valuable assistance of Luis Martarano in isolating the RNA. M.L.K. expresses her gratitude to Jim Garrison for his generosity in providing the computer program and for his help during the course of this study. We especially appreciate the support, comments, and enthusiasm Harvey Lodish has provided. This investigation was supported in part by grants from the National Science Foundation (PCM-8112215) and the March of Dimes (Basil O{\textquoteright}Connor Research Award) to M.L.K.",

year = "1985",

month = nov,

doi = "10.1016/0012-1606(85)90134-4",

language = "English (US)",

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pages = "203--212",

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T1 - Regional distribution of maternal messenger RNA in the amphibian oocyte

AU - King, Mary Lou

AU - Barklis, Eric

N1 - Funding Information: We thank Bob Power for expert technical assistance and unlimited patience in doing the cryostat sectioning. We acknowledge the valuable assistance of Luis Martarano in isolating the RNA. M.L.K. expresses her gratitude to Jim Garrison for his generosity in providing the computer program and for his help during the course of this study. We especially appreciate the support, comments, and enthusiasm Harvey Lodish has provided. This investigation was supported in part by grants from the National Science Foundation (PCM-8112215) and the March of Dimes (Basil O’Connor Research Award) to M.L.K.

PY - 1985/11

Y1 - 1985/11

N2 - We show that there is a non-random distribution of information in the form of mRNAs along the animal/vegetal axis of Xenopus oocytes. Polyadenylated RNA isolated from different regions along this axis was translated in a cell-free system, and the products were fractionated by two-dimensional gel electrophoresis. A comparison of the resulting protein products using a computer program has established that specific translatable mRNAs are regionally localized within the oocyte prior to fertilization. Seventeen mRNAs show significant qualitative or quantitative differences in their subcellular location. These sequences comprise as much as 4% of the total detected translatable polyadenylated RNA. Asymmetric segregation of these messages during Xenopus development may be involved in the determination of cell fate.

AB - We show that there is a non-random distribution of information in the form of mRNAs along the animal/vegetal axis of Xenopus oocytes. Polyadenylated RNA isolated from different regions along this axis was translated in a cell-free system, and the products were fractionated by two-dimensional gel electrophoresis. A comparison of the resulting protein products using a computer program has established that specific translatable mRNAs are regionally localized within the oocyte prior to fertilization. Seventeen mRNAs show significant qualitative or quantitative differences in their subcellular location. These sequences comprise as much as 4% of the total detected translatable polyadenylated RNA. Asymmetric segregation of these messages during Xenopus development may be involved in the determination of cell fate.

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DO - 10.1016/0012-1606(85)90134-4

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AN - SCOPUS:0022350576

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SP - 203

EP - 212

JO - Developmental Biology

JF - Developmental Biology

IS - 1

ER -

Regional distribution of maternal messenger RNA in the amphibian oocyte

Abstract

Funding

ASJC Scopus subject areas

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