TY - JOUR
T1 - Regulation of GluR1 abundance in murine hippocampal neurones by serum- and glucocorticoid-inducible kinase 3
AU - Strutz-Seebohm, Nathalie
AU - Seebohm, Guiscard
AU - Mack, Andreas F.
AU - Wagner, Hans Joachim
AU - Just, Lothar
AU - Skutella, Thomas
AU - Lang, Undine E.
AU - Henke, Guido
AU - Striegel, Marion
AU - Hollmann, Michael
AU - Rouach, Nathalie
AU - Nicoll, Roger A.
AU - McCormick, James A.
AU - Wang, Jian
AU - Pearce, David
AU - Lang, Florian
PY - 2005/6/1
Y1 - 2005/6/1
N2 - Phosphatidylinositol 3 kinase (PI3-kinase) is activated during and is required for hippocampal glutamate receptor-dependent long-term potentiation. It mediates the delivery of AMPA receptors to the neuronal surface. Among the downstream targets of PI3-kinase are three members of the serum- and glucocorticoid-inducible kinase family, SGK1, SGK2 and SGK3. In Xenopus oocytes expressing the AMPA subunit GluR1, we show that SGK3, and to a lesser extent SGK2, but not SGK1, increase glutamate-induced currents by increasing the abundance of GluR1 protein in the cell membrane. We further show Sgk3 mRNA expression in the hippocampus by RT-PCR and in situ hybridization. According to Western blotting, the hippocampal abundance of GluR1 is significantly lower in gene-targeted mice lacking SGK3 (Sgk3-/-) than in their wild-type littermates (Sgk3+/+). The present observations disclose a novel mechanism in the regulation of GluR1.
AB - Phosphatidylinositol 3 kinase (PI3-kinase) is activated during and is required for hippocampal glutamate receptor-dependent long-term potentiation. It mediates the delivery of AMPA receptors to the neuronal surface. Among the downstream targets of PI3-kinase are three members of the serum- and glucocorticoid-inducible kinase family, SGK1, SGK2 and SGK3. In Xenopus oocytes expressing the AMPA subunit GluR1, we show that SGK3, and to a lesser extent SGK2, but not SGK1, increase glutamate-induced currents by increasing the abundance of GluR1 protein in the cell membrane. We further show Sgk3 mRNA expression in the hippocampus by RT-PCR and in situ hybridization. According to Western blotting, the hippocampal abundance of GluR1 is significantly lower in gene-targeted mice lacking SGK3 (Sgk3-/-) than in their wild-type littermates (Sgk3+/+). The present observations disclose a novel mechanism in the regulation of GluR1.
UR - http://www.scopus.com/inward/record.url?scp=21044445830&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=21044445830&partnerID=8YFLogxK
U2 - 10.1113/jphysiol.2004.079582
DO - 10.1113/jphysiol.2004.079582
M3 - Article
C2 - 15774536
AN - SCOPUS:21044445830
SN - 0022-3751
VL - 565
SP - 381
EP - 390
JO - Journal of Physiology
JF - Journal of Physiology
IS - 2
ER -