Requirement for microtubules in new membrane formation during cytokinesis of Xenopus embryos

M. V. Danilchik; W. C. Funk; E. E. Brown; K. Larkin

doi:10.1006/dbio.1997.8815

Requirement for microtubules in new membrane formation during cytokinesis of Xenopus embryos

M. V. Danilchik, W. C. Funk, E. E. Brown, K. Larkin

Integrative Biosciences

Research output: Contribution to journal › Article › peer-review

108 Scopus citations

Abstract

In cleaving Xenopus eggs, exposure to nocodazole or cold shock prevents the addition of new plasma membrane to the cleavage plane and causes furrows to recede, suggesting a specific role for microtubules in cytokinesis. Whole- mount confocal immunocytochemistry reveals a ring of radially arranged, acetylated microtubule bundles at the base of all advancing cleavage furrows, from the first cleavage through the midblastula stage. We hypothesize that this novel microtubular structure is involved in transporting maternal stores of membrane in the subcortex to a site of membrane addition near the leading edge of the furrow.

Original language	English (US)
Pages (from-to)	47-60
Number of pages	14
Journal	Developmental Biology
Volume	194
Issue number	1
DOIs	https://doi.org/10.1006/dbio.1997.8815
State	Published - Feb 1 1998

Keywords

Cell division
Cleavage furrow
Confocal microscopy
Cytokinesis
Egg
Embryo
Membrane
Microtubule
Midbody
Midzone
Xenopu s

ASJC Scopus subject areas

Molecular Biology
Developmental Biology
Cell Biology

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10.1006/dbio.1997.8815

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abstract = "In cleaving Xenopus eggs, exposure to nocodazole or cold shock prevents the addition of new plasma membrane to the cleavage plane and causes furrows to recede, suggesting a specific role for microtubules in cytokinesis. Whole- mount confocal immunocytochemistry reveals a ring of radially arranged, acetylated microtubule bundles at the base of all advancing cleavage furrows, from the first cleavage through the midblastula stage. We hypothesize that this novel microtubular structure is involved in transporting maternal stores of membrane in the subcortex to a site of membrane addition near the leading edge of the furrow.",

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note = "Funding Information: This work was supported by NSF IBN-9396063 and 9631765.",

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AU - Danilchik, M. V.

AU - Funk, W. C.

AU - Brown, E. E.

AU - Larkin, K.

N1 - Funding Information: This work was supported by NSF IBN-9396063 and 9631765.

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KW - Cell division

KW - Cleavage furrow

KW - Confocal microscopy

KW - Cytokinesis

KW - Egg

KW - Embryo

KW - Membrane

KW - Microtubule

KW - Midbody

KW - Midzone

KW - Xenopu s

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JF - Developmental Biology

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Requirement for microtubules in new membrane formation during cytokinesis of Xenopus embryos

Abstract

Keywords

ASJC Scopus subject areas

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