Retinal optical coherence tomography at 1 μm with dynamic focus control and axial motion tracking

Michelle Cua, Sujin Lee, Dongkai Miao, Myeong Jin Ju, Paul J. Mackenzie, Yifan Jian, Marinko V. Sarunic

Research output: Contribution to journalArticlepeer-review

22 Scopus citations


High-resolution optical coherence tomography (OCT) retinal imaging is important to noninvasively visualize the various retinal structures to aid in better understanding of the pathogenesis of vision-robbing diseases. However, conventional OCT systems have a trade-off between lateral resolution and depth-of-focus. In this report, we present the development of a focus-stacking OCT system with automatic focus optimization for high-resolution, extended-focal-range clinical retinal imaging by incorporating a variable-focus liquid lens into the sample arm optics. Retinal layer tracking and selection was performed using a graphics processing unit accelerated processing platform for focus optimization, providing real-time layer-specific en face visualization. After optimization, multiple volumes focused at different depths were acquired, registered, and stitched together to yield a single, high-resolution focus-stacked dataset. Using this system, we show high-resolution images of the retina and optic nerve head, from which we extracted clinically relevant parameters such as the nerve fiber layer thickness and lamina cribrosa microarchitecture.

Original languageEnglish (US)
Article number026007
JournalJournal of biomedical optics
Issue number2
StatePublished - Feb 1 2016
Externally publishedYes


  • en face optical coherence tomography
  • focus-stacking
  • graphics processing unit acceleration
  • high-resolution optical coherence tomography
  • variable-focus lens

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering


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